US 11,965,196 B2
Enzymatic preparation method of inclusion complexes of tributyrin
Caiming Li, Wuxi (CN); Zhaofeng Li, Wuxi (CN); Shuangdi Chen, Wuxi (CN); Zhengbiao Gu, Wuxi (CN); Yan Feng, Wuxi (CN); Zexi Li, Wuxi (CN); Li Cheng, Wuxi (CN); and Yan Hong, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Sep. 28, 2020, as Appl. No. 17/034,234.
Application 17/034,234 is a continuation of application No. PCT/CN2018/089940, filed on Jun. 5, 2018.
Claims priority of application No. 2018103471067 (CN), filed on Apr. 18, 2018.
Prior Publication US 2021/0010044 A1, Jan. 14, 2021
Int. Cl. C12P 19/04 (2006.01); A23K 10/14 (2016.01); A23K 20/158 (2016.01); A23K 20/163 (2016.01); A23K 40/30 (2016.01); A61K 9/107 (2006.01); A61K 31/19 (2006.01); A61K 47/69 (2017.01); C12P 19/18 (2006.01)
CPC C12P 19/04 (2013.01) [A23K 10/14 (2016.05); A23K 20/158 (2016.05); A23K 20/163 (2016.05); A23K 40/30 (2016.05); A61K 9/107 (2013.01); A61K 31/19 (2013.01); A61K 47/6951 (2017.08); C12P 19/18 (2013.01)] 8 Claims
 
1. A method of enzymatic preparation of inclusion complexes of tributyrin, comprising:
(a) preparing starch milk or a maltodextrin solution as a raw material, wherein a mass concentration of the starch milk or maltodextrin solution is 15% to 30%, and wherein the starch milk is an aqueous suspension of starch from one or more of corn starch, waxy corn starch, potato starch, rice starch, cassava starch, and wheat starch;
(b) adding an enzyme into the starch milk or maltodextrin solution for liquefaction reaction and cyclization reaction to form cyclodextrin, wherein the enzyme is cyclodextrin glucosyltransferase which catalyzes cyclization of glucan polymers in the starch milk to form the cyclodextrin, and wherein liquefaction is performed at a temperature of 80° C. to 90° C.; and
(c) adding tributyrin and an emulsifier so that tributyrin is included,
wherein there is no separation and/or purification of the cyclodextrin between steps (b) and (c).