US 11,965,171 B2
Plants having increased tolerance to herbicides
Stefan Tresch, Ludwigshafen (DE); Doreen Schachtschabel, Ludwigshafen (DE); Mihiret Tekeste Sisay, Ludwigshafen (DE); Jens Lerchl, Limburgerhof (DE); Julia Major, Freinsheim (DE); Florian Vogt, Ludwigshafen (DE); Frederick Calo, Dusseldorf (DE); and Jill Marie Paulik, Durham, NC (US)
Assigned to BASF SE, Ludwigshafen (DE)
Filed by BASF SE, Ludwigshafen (DE)
Filed on Jun. 16, 2022, as Appl. No. 17/842,427.
Application 17/842,427 is a continuation of application No. 16/824,787, filed on Mar. 20, 2020, granted, now 11,365,426.
Application 16/824,787 is a continuation of application No. 15/305,931, granted, now 10,597,673, previously published as PCT/EP2015/058633, filed on Apr. 22, 2015.
Claims priority of provisional application 61/982,903, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,904, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,894, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,900, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,895, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,901, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,897, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,899, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,898, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,896, filed on Apr. 23, 2014.
Claims priority of provisional application 61/982,893, filed on Apr. 23, 2014.
Prior Publication US 2022/0333127 A1, Oct. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); A01H 4/00 (2006.01); A01N 43/68 (2006.01); C12N 9/10 (2006.01)
CPC C12N 15/8274 (2013.01) [A01H 4/008 (2013.01); A01N 43/68 (2013.01); C12N 9/1059 (2013.01); C12Y 204/01012 (2013.01)] 10 Claims
 
1. A plant or plant part comprising a polynucleotide encoding a mutated cellulose synthase (CESA) polypeptide, the expression of said polynucleotide confers to the plant or plant part tolerance to CESA-inhibiting herbicides, wherein the mutated CESA polypeptide comprises one or more of the following motifs:
i) Motif 1a:
(SEQ ID NO: 78)
[V/I][A/V]G[V/I/F][S/T][Y/D/N/A]A[V/I/L][N/S/G]
 
[S/N]G[Y/F/E][Q/D/G/E/H][S/A]WG[P/A]L[[F/M/L]G
 
[K/R][L/V][F/L]F,
wherein the amino acid at position 5 within said motif is substituted by any other amino acid than S or T;
wherein the amino acid at position 16 within said motif is substituted by any other amino acid; and/or
wherein the amino acid at position 17 within said motif is substituted by any other amino acid than P or A;
ii) Motif 2a:
(SEQ ID NO: 81)
[V/L/I]W[S/A][V/A/I]LL[A/S]S[I/F/V][F/L][S/T][L/V]
 
[L/M/V/I]WV[R/K][I/V][N/D]PF,
 wherein the amino acid at position 8 within said motif is substituted by any other amino acid, and/or position 11 by any other amino acid than S or T;
wherein the mutated CESA polypeptide comprises an amino acid sequence having, over the full-length, at least 80% sequence identity to SEQ ID NO: 28, and wherein the amino acid sequence of the mutated CESA polypeptide differs from the wildtype amino acid sequence at one or more positions corresponding to positions 1009, 1010, 1013, 1052, and 1055 of SEQ ID NO: 1.