US 11,965,168 B2
Leghemoglobin in soybean
Hyeon-Je Cho, Ankeny, IA (US); John D Everard, Grimes, IA (US); Anthony J Kinney, Wilmington, DE (US); Zhan-Bin Liu, Clive, IA (US); Knut Meyer, Wilmington, DE (US); Thomas G Patterson, Westfield, IN (US); Kevin G Ripp, Des Moines, IA (US); and Bo Shen, Johnston, IA (US)
Assigned to PIONEER HI-BRED INTERNATIONAL, INC., , IA (US)
Filed by PIONEER HI-BRED INTERNATIONAL, INC., Johnston, IA (US)
Filed on Jun. 30, 2022, as Appl. No. 17/810,000.
Application 17/810,000 is a continuation of application No. 17/451,913, filed on Oct. 22, 2021, abandoned.
Claims priority of provisional application 63/106,519, filed on Oct. 28, 2020.
Prior Publication US 2022/0340923 A1, Oct. 27, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01); A01H 1/00 (2006.01); A01H 5/10 (2018.01); A01H 6/54 (2018.01)
CPC C12N 15/8251 (2013.01) [C07K 14/415 (2013.01); A01H 1/101 (2021.01); A01H 5/10 (2013.01); A01H 6/542 (2018.05)] 20 Claims
OG exemplary drawing
 
1. A soybean seed comprising a leghemoglobin protein in an amount of at least 0.5% of total protein in the soybean seed, a first genomic modification and a second genomic modification, wherein the first genomic modification comprises an insertion of a leghemoglobin coding sequence into a native seed storage protein gene encoding a glycinin or a conglycinin, and wherein the insertion does not include a signal peptide sequence, and wherein the second genomic modification reduces expression of a seed storage protein coding sequence at a genomic locus different from the first genomic modification.