CPC C12N 15/8251 (2013.01) [C07K 14/415 (2013.01); A01H 1/101 (2021.01); A01H 5/10 (2013.01); A01H 6/542 (2018.05)] | 20 Claims |
1. A soybean seed comprising a leghemoglobin protein in an amount of at least 0.5% of total protein in the soybean seed, a first genomic modification and a second genomic modification, wherein the first genomic modification comprises an insertion of a leghemoglobin coding sequence into a native seed storage protein gene encoding a glycinin or a conglycinin, and wherein the insertion does not include a signal peptide sequence, and wherein the second genomic modification reduces expression of a seed storage protein coding sequence at a genomic locus different from the first genomic modification.
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