US 11,965,023 B2
Anti-Siglec-5 antibodies and methods of use thereof
Kate Monroe, Berkeley, CA (US); Helen Lam, Union City, CA (US); Patricia Culp, Oakland, CA (US); and Arnon Rosenthal, Woodside, CA (US)
Assigned to Alector LLC, South San Francisco, CA (US)
Filed by Alector LLC, South San Francisco, CA (US)
Filed on May 9, 2022, as Appl. No. 17/740,100.
Application 17/740,100 is a division of application No. 16/614,302, granted, now 11,359,014, previously published as PCT/US2018/032780, filed on May 15, 2018.
Claims priority of provisional application 62/506,789, filed on May 16, 2017.
Prior Publication US 2022/0315656 A1, Oct. 6, 2022
Int. Cl. C07K 16/28 (2006.01); A61K 39/00 (2006.01); A61P 25/00 (2006.01); A61P 25/08 (2006.01); A61P 25/28 (2006.01); A61P 29/00 (2006.01); A61P 31/04 (2006.01); A61P 35/00 (2006.01); A61P 37/00 (2006.01)
CPC C07K 16/2803 (2013.01) [A61P 25/00 (2018.01); A61P 25/08 (2018.01); A61P 25/28 (2018.01); A61P 29/00 (2018.01); A61P 31/04 (2018.01); A61P 35/00 (2018.01); A61P 37/00 (2018.01); A61K 2039/505 (2013.01); C07K 2317/33 (2013.01); C07K 2317/34 (2013.01); C07K 2317/56 (2013.01); C07K 2317/567 (2013.01); C07K 2317/73 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01)] 32 Claims
 
1. A method of preventing, reducing risk, or treating a disease, disorder, or injury selected from the group consisting of Alzheimer's disease and cancer, comprising administering to an individual in need thereof a therapeutically effective amount of an anti-Siglec-5 antibody that decreases cellular levels of Siglec-5, inhibits interaction between Siglec-5 and one or more Siglec-5 ligands, or both;
wherein the cancer expresses Siglec-5 or one or more Siglec-5 ligands;
wherein the anti-Siglec-5 antibody comprises a light chain variable region comprising an HVR-L1, HVR-L2, and HVR-L3 and a heavy chain variable region comprising an HVR-H1, HVR-H2, and HVR-H3, and wherein:
a. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 3, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 13, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 20, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 27, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 35, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 45;
b. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 4, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 14, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 21, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 27, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 36, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 46;
c. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 5, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 14, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 22, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 28, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 37, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 47;
d. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 6, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 14, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 23, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 27, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 38, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 46;
e. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 4, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 14, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 24, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 29, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 39, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 48;
f. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 7, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 15, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 30, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 40, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 49;
g. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 7, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 15, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 31, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 41, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 50;
h. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 8, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 16, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 32, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 42, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 51;
i. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 9, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 16, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 31, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 41, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 50;
j. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 10, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 16, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 33, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 40, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 51;
k. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 11, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 14, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 22, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 28, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 37, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 47;
l. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 12, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 17, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 26, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 34, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 43, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 52;
m. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 11, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 18, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 24, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 27, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 44, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 53; or
n. the HVR-L1 comprises the amino acid sequence of SEQ ID NO: 9, the HVR-L2 comprises the amino acid sequence of SEQ ID NO: 19, the HVR-L3 comprises the amino acid sequence of SEQ ID NO: 25, the HVR-H1 comprises the amino acid sequence of SEQ ID NO: 33, the HVR-H2 comprises the amino acid sequence of SEQ ID NO: 40, and the HVR-H3 comprises the amino acid sequence of SEQ ID NO: 54.