CPC B01L 3/502761 (2013.01) [B01L 3/502715 (2013.01); G01N 33/5094 (2013.01); G06T 7/0012 (2013.01); B01L 2200/0663 (2013.01); B01L 2300/0654 (2013.01); G06T 2207/30024 (2013.01)] | 13 Claims |
1. A method for determining a sepsis condition of a subject comprising:
receiving a dataset generated from a blood sample of the subject, wherein the dataset comprises, for each leukocyte of the blood sample, a plurality of images of the leukocyte as the leukocyte is deformed within a deformation region of a microfluidic channel;
filtering the dataset to exclude images comprising overlapping leukocytes, comprising detecting the overlapping leukocytes based on a monotonicity in feature motion between images of the leukocyte;
determining a biophysical parameter dataset by, for each leukocyte of the blood sample:
determining one or more biophysical parameter associated with the respective leukocyte based on the plurality of images associated with the respective leukocyte;
linearly adjusting the one or more biophysical parameters based on at least one of a flow rate and a path traversed by the respective leukocyte; and
determining a numerical index based on the biophysical parameter dataset; and
based on the numerical index, determining a probability that the subject is experiencing sepsis.
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