US 11,964,279 B2
Sample preparation cartridges and apparatuses
Manojkumar M. Nair, Burlington, MA (US); and John Frederick Regan, Boxford, MA (US)
Assigned to LexaGene, Inc., Beverly, MA (US)
Appl. No. 17/626,885
Filed by LexaGene, Inc., Beverly, MA (US)
PCT Filed Jul. 14, 2020, PCT No. PCT/US2020/041875
§ 371(c)(1), (2) Date Jan. 13, 2022,
PCT Pub. No. WO2021/011512, PCT Pub. Date Jan. 21, 2021.
Claims priority of provisional application 62/874,212, filed on Jul. 15, 2019.
Prior Publication US 2022/0258162 A1, Aug. 18, 2022
Int. Cl. B01L 3/00 (2006.01); C12N 1/06 (2006.01); C12N 15/10 (2006.01); G01N 33/543 (2006.01); G01N 35/00 (2006.01); G01N 35/10 (2006.01)
CPC B01L 3/502753 (2013.01) [C12N 1/06 (2013.01); C12N 15/1017 (2013.01); G01N 33/54326 (2013.01); B01L 2200/0605 (2013.01); B01L 2300/0681 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/0622 (2013.01); G01N 2035/00564 (2013.01); G01N 2035/1032 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A sample preparation cartridge, comprising:
a lysis chamber, comprising:
a filter partitioning the lysis chamber into a first lysis chamber section and a second lysis chamber section;
a first lysis chamber port in fluidic connectivity with the first lysis chamber section;
a second lysis chamber port in fluidic connectivity with the first lysis chamber section; and
a means for lysing; and
a nucleic acid capture chamber, comprising:
a fluidic channel with an inlet and an outlet, wherein the inlet is in fluidic connectivity with the second lysis chamber section;
a nucleic acid capture chamber port in fluidic connectivity with the outlet of the fluidic channel; and
a nucleic acid binding unit in the fluidic channel; and
wherein the first lysis chamber port is capable of establishing fluidic connectivity with a fluid comprising nucleic acids, the second lysis chamber port is capable of establishing fluidic connectivity with at least one fluidic movement source capable of flowing the fluid to the first lysis chamber section, the nucleic acid capture chamber port is capable of establishing fluidic connectivity with at least one fluidic movement source capable of flowing the fluid in the second lysis chamber section through the fluidic channel.