US 11,963,993 B2
Process for obtaining a tannin extract isolated from grapes, tannin extract obtained and uses thereof
Bacardit Dalmases Anna, Igualada (ES); Ollé Otero Luis, Igualada (ES); Sorolla Casellas Sílvia, Igualada (ES); and Casas Solé Concepció, Igualada (ES)
Assigned to Universitat de Lleida, Lleida (ES)
Appl. No. 16/303,915
Filed by UNIVERSITAT DE LLEIDA, Lleida (ES)
PCT Filed May 23, 2017, PCT No. PCT/IB2017/053035
§ 371(c)(1), (2) Date Nov. 21, 2018,
PCT Pub. No. WO2017/203429, PCT Pub. Date Nov. 30, 2017.
Claims priority of application No. ES201630673 (ES), filed on May 24, 2016.
Prior Publication US 2020/0121752 A1, Apr. 23, 2020
Int. Cl. A61K 36/87 (2006.01); A23L 33/105 (2016.01); A61K 8/9789 (2017.01); A61Q 19/00 (2006.01); C14C 3/00 (2006.01); C14C 3/08 (2006.01); C14C 3/12 (2006.01)
CPC A61K 36/87 (2013.01) [A23L 33/105 (2016.08); A61K 8/9789 (2017.08); A61Q 19/00 (2013.01); C14C 3/00 (2013.01); C14C 3/08 (2013.01); C14C 3/12 (2013.01); A61K 2800/10 (2013.01); A61K 2800/43 (2013.01); A61K 2800/522 (2013.01)] 15 Claims
 
1. A tannin extract obtained by a method comprising:
(a) adding an aqueous solution of a polyphenol-solubilizing agent to whole grape seeds without grinding or milling, wherein the polyphenol-solubilizing agent is selected from the group consisting of urea, sodium hydroxide, and a compound containing an SO3 group and is present at a concentration in the aqueous solution of 1-15% by weight with respect to the weight of the whole grape seeds;
(b) reacting the aqueous solution of the polyphenol-solubilizing agent and the whole grape seeds at a pressure ranging between 2.5 and 3.5 atmospheres, and a temperature ranging between 100° C. and 160° C., in a period of time ranging from 1-2 hours in an autoclave reactor; and
(c) recovering a liquid extract from the autoclave reactor,
wherein the liquid extract comprises a mixture of pure hydrolysable tannins, pure condensed tannins that include procyanidins, and non-tannins that include gallic acid, and further wherein the liquid extract has a content of tannins or polyphenols that is greater than 50%.