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1. An in vitro expansion process for rapid of antigen specific T cells, comprising culturing in a vessel comprising a gas permeable culture surface a population of PBMCs in a culture in the presence of an antigen, wherein the antigen is in the form of a peptide or a peptide mix relevant to a target antigen(s) and wherein the antigen is added to the culture in the range of from 1 to 500 ng per ml; wherein the culturing is performed in the presence of an exogenous cytokine wherein the cytokine is other than exogenous IL-2 and is selected from the group comprising IL-1, IL-4, IL-6, IL-7, IL-12, IL-15 and combinations thereof; wherein antigens, cytokines, media and nutrients are not added or changed after initiation of the expansion process; wherein the expansion to provide the desired population of T cells is 20 days or less; wherein the desired population of cells is a CD56− population; and wherein the peptide or peptide mix is/are not added to the culture presented on dendritic cells.
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