US 11,963,542 B2
Casein micelle with composition and structural features simulating human milk and preparation method thereof
Peng Zhou, Wuxi (CN); Dasong Liu, Wuxi (CN); Tingting Yang, Wuxi (CN); and Lina Zhang, Wuxi (CN)
Assigned to JIANGNAN UNIVERSITY, Wuxi (CN)
Filed by Jiangnan University, Wuxi (CN)
Filed on Mar. 13, 2023, as Appl. No. 18/120,628.
Application 18/120,628 is a continuation of application No. PCT/CN2022/093268, filed on May 17, 2022.
Claims priority of application No. 202210149999.0 (CN), filed on Feb. 10, 2022.
Prior Publication US 2023/0210156 A1, Jul. 6, 2023
Int. Cl. A23L 33/19 (2016.01); A23J 1/20 (2006.01); A23J 3/10 (2006.01); A23L 33/00 (2016.01)
CPC A23L 33/19 (2016.08) [A23J 1/202 (2013.01); A23J 3/10 (2013.01); A23L 33/40 (2016.08)] 12 Claims
OG exemplary drawing
 
1. A method for preparing a casein micelle simulating human milk, comprising the following steps:
adding three ions involving a citrate ion, a calcium ion, and a phosphate ion to a solution of compound casein to obtain a mixed solution; and if the pH of the mixed solution is below the range of 6.7-7.4, adjusting the pH of the mixed solution to 6.7-7.4 by adding a NaOH solution or a KOH solution, and obtaining a solution of the casein micelle simulating human milk;
wherein β-casein, κ-casein, and αs1-casein have a mass proportion of 65-71%, 17-23%, and 9-15% in the compound casein respectively; and
in the solution of the casein micelle simulating human milk, the compound casein has a mass concentration of 0.3-0.6%, the citrate ion has a concentration of 1.5-4.2 mM, the calcium ion has a concentration of 4.5-10.8 mM, and the phosphate ion has a concentration of 1.1-4.5 mM.