	US 11,963,519 B2
	Kit and method for constructing long-acting depression animal model
Cen Li, Xining (CN); Hongxia Yang, Xining (CN); Jing Zhao, Xining (CN); Lixin Wei, Xining (CN); Yuzhi Du, Xining (CN); Yuancan Xiao, Xining (CN); Hongtao Bi, Xining (CN); Zhanjuan Chen, Xining (CN); Yajun Qiao, Xining (CN); and Jianv Wang, Xining (CN)
Assigned to Northwest Institute of Plateau Biology, CAS, Xining (CN)
Filed by Northwest Institute of Plateau Biology, CAS, Xining (CN)
Filed on Aug. 25, 2022, as Appl. No. 17/895,405.
Claims priority of application No. 202210384349.4 (CN), filed on Apr. 13, 2022.
Prior Publication US 2023/0329200 A1, Oct. 19, 2023
Int. Cl. A01K 67/02 (2006.01); C07K 14/235 (2006.01); C07K 14/35 (2006.01)

CPC A01K 67/02 (2013.01) [C07K 14/235 (2013.01); C07K 14/35 (2013.01); A01K 2227/105 (2013.01); A01K 2267/0356 (2013.01)]

5 Claims

1. A method for constructing a long-acting depression animal model, comprising a step of administering Bacille Calmette-Guerin (BCG) and pertussis toxin (PTX) for combined induction, comprising:

intraperitoneally injecting 50 μL to 400 μL of a working solution of BCG into a mouse on day 0, and intraperitoneally injecting 50 μL to 400 μL of a working solution of PTX into the mouse on day 0 to day 1 and day 2 to day 3;

wherein the working solution of BCG has an M. bovis CFU concentration of 1×10⁶to 6×10⁷CFU/mL; and

the working solution of PTX has a concentration of 0.5 μg/mL to 4 μg/mL.