	US 11,963,491 B2
	Method for enriching lutein in broccoli sprouts by γ-aminobutyric acid combined with sodium chloride stress
Dajing Li, Jiangsu (CN); Weiwei He, Jiangsu (CN); Zhiyi Zhou, Jiangsu (CN); Yadong Xiao, Jiangsu (CN); Jiangfeng Song, Jiangsu (CN); and Zhongyuan Zhang, Jiangsu (CN)
Assigned to Jiangsu Academy Of Agricultural Sciences, Jiangsu (CN)
Appl. No. 17/257,300
Filed by JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, Jiangsu (CN)
PCT Filed Oct. 26, 2020, PCT No. PCT/CN2020/123608 § 371(c)(1), (2) Date Dec. 30, 2020, PCT Pub. No. WO2021/232674, PCT Pub. Date Nov. 25, 2021.
Claims priority of application No. 202010438549.4 (CN), filed on May 21, 2020.
Prior Publication US 2022/0110267 A1, Apr. 14, 2022
Int. Cl. A01C 1/00 (2006.01); A01C 1/08 (2006.01); A01G 7/06 (2006.01); A01G 31/00 (2018.01)

CPC A01G 7/06 (2013.01) [A01C 1/08 (2013.01); A01G 31/00 (2013.01)]

2 Claims

1. A method for enriching lutein in broccoli sprouts by γ-aminobutyric acid combined with sodium chloride stress, comprising the following steps:

selecting broccoli seeds as raw materials,

removing impurities in the broccoli seeds,

subjecting the broccoli seeds to disinfection using a sodium hypochlorite solution to obtain disinfected seeds,

subjecting the disinfected seeds to soaking treatment to obtain soaked seeds,

subjecting the soaked seeds to germination for one day to obtain germinated seeds, and

spraying 20 mL of a mixed aqueous solution of NaCl and γ-aminobutyric acid onto the germinated seeds every 12 h for four days, to obtain broccoli sprouts rich in lutein, wherein a concentration of NaCl in the mixed aqueous solution is 100 mM, and a concentration of γ-aminobutyric acid in the mixed aqueous solution is 0.25-1.00 mmol/L.