US 11,947,098 B2
Multi-focal light-sheet structured illumination fluorescence microscopy system
Chrysanthe Preza, Memphis, TN (US); Ana Doblas, Memphis, TN (US); Genaro Saavedra-Tortosa, Valencia (ES); Manuel Martinez-Corral, Valencia (ES); and Juan Carlos Barreiro, Valencia (ES)
Filed by Chrysanthe Preza, Memphis, TN (US); Ana Doblas, Memphis, TN (US); Genaro Saavedra-Tortosa, Valencia (ES); Manuel Martinez-Corral, Valencia (ES); and Juan Carlos Barreiro, Valencia (ES)
Filed on Mar. 29, 2022, as Appl. No. 17/707,628.
Application 17/707,628 is a continuation of application No. 16/024,807, filed on Jun. 30, 2018, granted, now 11,287,627.
Claims priority of provisional application 62/527,232, filed on Jun. 30, 2017.
Prior Publication US 2022/0291495 A1, Sep. 15, 2022
Int. Cl. G02B 21/00 (2006.01)
CPC G02B 21/0076 (2013.01) [G02B 21/0032 (2013.01); G02B 21/0064 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A structured illumination system for a fluorescence microscope, comprising:
a first linear polarizer configured to receive a light beam;
a plurality of equidistant parallel slits configured to be illuminated by the light beam after passing through the first polarizer;
a Wollaston prism comprising two birefringent wedges, each birefringent wedge with an optical axis, configured to divide the light beam into two spherical waves to generate a plurality of light-sheet structured patterns;
a first converging lens with a front focal plane, configured to receive the divided light beam after passing through the Wollaston prism, wherein the front focal plane is collinear with the plurality of equidistant parallel slits; and
a second linear polarizer configured to receive the light beam after passing through the first converging lens but prior to illuminating a sample.