CPC G01N 15/10 (2013.01) [B01L 3/502753 (2013.01); C12N 5/061 (2013.01); C12N 5/0612 (2013.01); G01N 1/4005 (2013.01); G01N 15/00 (2013.01); C12M 21/06 (2013.01); G01N 2001/4088 (2013.01); G01N 2015/0003 (2013.01); G01N 2015/0065 (2013.01); G01N 2015/1006 (2013.01); G01N 2015/1081 (2013.01); G01N 2800/367 (2013.01)] | 30 Claims |
1. A method for sorting sperm, comprising:
delivering a sample of sperm to an inlet of a fluidic system;
allowing some sperm from the sample of sperm to traverse a flow path in a flow-free manner through the fluidic system toward an outlet of a filter chamber of the fluidic system;
filtering the sperm within the filter chamber using a filter and gravity to restrict movement of the sperm through the filter, wherein the filter has micropores sized to permit a head of the sperm in the sample to pass therethrough;
allowing the sperm after passing through the filter and overcoming gravity, to pass in a flow-free manner through the outlet and into an additional collection channel and/or chamber arranged at the outlet such that it is in fluid communication with the filter chamber and such that a percentage of motile sperm that are present at the additional collection channel and/or chamber is greater than a percentage in the sample; and
harvesting sperm in a liquid from the additional collection channel and/or chamber to retrieve the harvested sperm from the fluidic system, wherein the harvested sperm comprise some live, functional sperm from the sample.
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