	US 11,946,079 B2
	Method for producing a protein hydrolysate using an endopeptidase and a carboxypeptidase
Hanne Vang Hendriksen, Holte (DK); Gitte Budolfsen Lynglev, Frederiksberg (DK); Henrik Frisner, Frederiksberg (DK); Ciu Liu, Beijing (CN); Ye Liu, Beijing (CN); Eduardo Antonio Della Pia, Lyngby (DK); Hans Peter Heldt-Hansen, Virum (DK); Kenneth Jensen, Oelsted (DK); Wei Peng, Beijing (DK); and Ming Li, Beijing (CN)
Assigned to Novozymes A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on Jan. 10, 2022, as Appl. No. 17/571,594.
Application 17/571,594 is a division of application No. 16/611,539, granted, now 11,254,919, previously published as PCT/CN2018/090425, filed on Jun. 8, 2018.
Claims priority of application No. PCT/CN2017/087723 (WO), filed on Jun. 9, 2017.
Prior Publication US 2022/0127592 A1, Apr. 28, 2022
Int. Cl. C12N 9/48 (2006.01); C12P 21/06 (2006.01)

CPC C12N 9/485 (2013.01) [C12P 21/06 (2013.01)]

3 Claims

1. A method for producing a protein hydrolysate comprising:

a. providing an aqueous solution or suspension of a protein substrate; and

b. exposing the aqueous solution or suspension of the protein substrate to a polypeptide having endopeptidase activity and to a polypeptide having carboxypeptidase activity, to obtain the protein hydrolysate;

wherein the polypeptide having carboxypeptidase activity comprises an amino acid sequence with at least 90% sequence identity to SEQ ID Nos: 2, 4, 6, 8, or 10 and also is characterised by having a Pro/ACHA*100 ratio of at least 30.