US 11,944,679 B2
Genome-wide identification of immune evasion functions in a virus
Yushen Du, Los Angeles, CA (US); Nicholas C. Wu, Los Angeles, CA (US); and Ren Sun, Pacific Palisades, CA (US)
Assigned to THE REGENTS OF THE UNIVERSITY OF CALIFORNIA, Oakland, CA (US)
Appl. No. 16/493,441
Filed by The Regents of the University of California, Oakland, CA (US)
PCT Filed Mar. 14, 2018, PCT No. PCT/US2018/022465
§ 371(c)(1), (2) Date Sep. 12, 2019,
PCT Pub. No. WO2018/170147, PCT Pub. Date Sep. 20, 2018.
Claims priority of provisional application 62/471,269, filed on Mar. 14, 2017.
Prior Publication US 2020/0129612 A1, Apr. 30, 2020
Int. Cl. A61K 39/145 (2006.01); C07K 14/005 (2006.01); C12N 7/00 (2006.01); C12N 15/10 (2006.01)
CPC A61K 39/145 (2013.01) [C07K 14/005 (2013.01); C12N 7/00 (2013.01); C12N 15/1058 (2013.01); C12N 15/1072 (2013.01); C12N 2760/16121 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16162 (2013.01)] 9 Claims
 
1. A composition of matter, comprising:
a genetically engineered influenza A virus genome comprises a combination of nucleotide mutations at more than two amino acid positions selected from:
at least one amino acid substitution in SEQ ID NO: 3 at amino acid position N9, Q75 or T76; and/or
at least one amino acid substitution in SEQ ID NO: 5 at amino acid position N36, R72 or S225.