CPC G06V 10/34 (2022.01) [G06V 20/695 (2022.01)] | 20 Claims |
1. A method of removing contributions of autofluorescence in one or more channels of a multichannel image of a biological sample stained with one or more fluorescent labels, the method comprising:
independently calculating a contribution of autofluorescence in each channel of each pixel of the multichannel image based: (i) on one or more retrieved autofluorescence spectra; and (ii) normalized measured spectra for pixels of the multichannel image, and
subtracting from each measured pixel spectrum of the multichannel image the calculated contribution of autofluorescence in each channel for that respective pixel.
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