US 11,941,861 B2
Systems and methods for computing the contributions of autofluorescence in multichannel image
Joerg Bredno, Tucson, AZ (US); Mehrnoush Khojasteh, Tucson, AZ (US); Ilya Ravkin, Tucson, AZ (US); Smadar Shiffman, Tucson, AZ (US); and Xingwei Wang, Tucson, AZ (US)
Assigned to VENTANA MEDICAL SYSTEMS, INC., Tucson, AZ (US)
Filed by VENTANA MEDICAL SYSTEMS, INC., Tucson, AZ (US)
Filed on Aug. 6, 2021, as Appl. No. 17/444,624.
Application 17/444,624 is a continuation of application No. PCT/US2020/017436, filed on Feb. 10, 2020.
Claims priority of provisional application 62/805,239, filed on Feb. 13, 2019.
Prior Publication US 2021/0397870 A1, Dec. 23, 2021
Int. Cl. G01N 21/64 (2006.01); G06V 10/34 (2022.01); G06V 20/69 (2022.01)
CPC G06V 10/34 (2022.01) [G06V 20/695 (2022.01)] 20 Claims
OG exemplary drawing
 
1. A method of removing contributions of autofluorescence in one or more channels of a multichannel image of a biological sample stained with one or more fluorescent labels, the method comprising:
independently calculating a contribution of autofluorescence in each channel of each pixel of the multichannel image based: (i) on one or more retrieved autofluorescence spectra; and (ii) normalized measured spectra for pixels of the multichannel image, and
subtracting from each measured pixel spectrum of the multichannel image the calculated contribution of autofluorescence in each channel for that respective pixel.