US 11,940,442 B2
Method and system for determining integrated metabolic baseline and potential of living cells
David A. Ferrick, El Macero, CA (US); and Brian Dranka, Acton, MA (US)
Assigned to Agilent Technologies, Inc., Santa Clara, CA (US)
Filed by AGILENT TECHNOLOGIES, INC., Santa Clara, CA (US)
Filed on Jun. 30, 2020, as Appl. No. 16/917,352.
Application 16/917,352 is a continuation of application No. 15/082,658, filed on Mar. 28, 2016, abandoned.
Claims priority of provisional application 62/139,432, filed on Mar. 27, 2015.
Prior Publication US 2020/0333327 A1, Oct. 22, 2020
Int. Cl. G01N 33/50 (2006.01)
CPC G01N 33/5091 (2013.01) [G01N 33/5005 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of determining a single metabolic potential of a cell sample that is representative of both mitochondrial respiration and glycolysis, the method comprising the steps of:
measuring an initial oxygen consumption rate and an initial extracellular acidification rate of the cell sample;
thereafter, simultaneously administering to the cell sample a mitochondrial uncoupling agent and an ATP synthase inhibitor;
thereafter, simultaneously measuring a subsequent oxygen consumption rate and a subsequent extracellular acidification rate of the cell sample; and
determining a single metabolic potential of the cell sample that is representative of both mitochondrial respiration and glycolysis, wherein the single metabolic potential is plotted graphically where the Y axis represents oxygen consumption rate and the X axis represents extracellular acidification rate.