CPC G01N 33/5091 (2013.01) [G01N 33/5005 (2013.01)] | 15 Claims |
1. A method of determining a single metabolic potential of a cell sample that is representative of both mitochondrial respiration and glycolysis, the method comprising the steps of:
measuring an initial oxygen consumption rate and an initial extracellular acidification rate of the cell sample;
thereafter, simultaneously administering to the cell sample a mitochondrial uncoupling agent and an ATP synthase inhibitor;
thereafter, simultaneously measuring a subsequent oxygen consumption rate and a subsequent extracellular acidification rate of the cell sample; and
determining a single metabolic potential of the cell sample that is representative of both mitochondrial respiration and glycolysis, wherein the single metabolic potential is plotted graphically where the Y axis represents oxygen consumption rate and the X axis represents extracellular acidification rate.
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