US 11,940,427 B2
Liquid chromatography—stream equivalence by single stream calibration
Benjamin Tiemann, Munich (DE); Stefan Quint, Munich (DE); and Florian Schweinberger, Munich (DE)
Assigned to Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed by Roche Diagnostics Operations, Inc., Indianapolis, IN (US)
Filed on Sep. 20, 2021, as Appl. No. 17/448,063.
Claims priority of application No. 20204420 (EP), filed on Oct. 28, 2020.
Prior Publication US 2022/0128531 A1, Apr. 28, 2022
Int. Cl. G01N 30/72 (2006.01); G01N 30/02 (2006.01); G01N 30/88 (2006.01)
CPC G01N 30/7266 (2013.01) [G01N 30/88 (2013.01); G01N 2030/027 (2013.01)] 17 Claims
OG exemplary drawing
 
1. A liquid chromatography-mass spectrometry (LC-MS) apparatus comprising:
an ionization source coupled to a mass spectrometer;
a liquid chromatographic (LC) system coupled to the ionization source, wherein the LC system comprises multiple fluidic streams alternately connectable to the ionization source, thereby assigning a detection time window to each fluidic stream from the multiple fluidic streams when connected to the ionization source,
wherein the apparatus further comprises a controller that is configured to carry out steps of:
monitoring an ionization current of the ionization source for the multiple fluidic streams;
identifying differences in flow conditions between the multiple fluidic streams based on the monitored ionization current;
adjusting detection conditions of one or more of the multiple fluidic streams responsive to the identified differences, thereby enabling eluates of interest from each fluidic stream to be detected by the mass spectrometer in the respective detection time window.