US 11,940,410 B2
Functionalized nanopipette biosensor
Miloslav Karhanek, Santa Cruz, CA (US); Chris David Webb, Watsonville, CA (US); Senkei Umehara, Tokyo (JP); and Nader Pourmand, San Mateo, CA (US)
Assigned to The Regents of the University of California, Oakland, CA (US)
Filed by The Regents of the University of California, Oakland, CA (US)
Filed on Jan. 18, 2022, as Appl. No. 17/578,151.
Application 17/578,151 is a continuation of application No. 16/588,326, filed on Sep. 30, 2019, granted, now 11,255,814.
Application 16/588,326 is a continuation of application No. 16/298,232, filed on Mar. 11, 2019, abandoned.
Application 16/298,232 is a continuation of application No. 15/697,289, filed on Sep. 6, 2017, abandoned.
Application 15/697,289 is a continuation of application No. 14/603,134, filed on Jan. 22, 2015, granted, now 9,766,204, issued on Sep. 19, 2017.
Application 14/603,134 is a continuation of application No. 12/435,056, filed on May 4, 2009, granted, now 8,940,142, issued on Jan. 27, 2015.
Claims priority of provisional application 61/126,644, filed on May 5, 2008.
Prior Publication US 2022/0260520 A1, Aug. 18, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 27/42 (2006.01); G01N 27/403 (2006.01); G01N 33/487 (2006.01)
CPC G01N 27/42 (2013.01) [G01N 27/4035 (2013.01); G01N 33/48707 (2013.01)] 13 Claims
OG exemplary drawing
 
1. A device for specific detection of a plurality of analytes in a sample, the device comprising:
a flow chamber comprising:
a plurality of capillary nanopipettes,
each capillary nanopipette comprising:
a tip with a 10 nm-100 nm diameter, wherein the tip is positioned in the flow chamber for contacting a sample flowing through the flow chamber;
a coating of an analyte-binding peptide at the tip, wherein the analyte-binding peptide specifically binds to an analyte;
an electrode positioned in an interior volume of the capillary nanopipette and in contact with an electrolyte solution contained in the interior volume,
a current detecting circuit connected to the electrode in each capillary nanopipette for detecting current through the tip;
an electrode positioned in the flow chamber outside of the nanopipettes for contacting the sample and connected to the current detecting circuit;
an amplifier connected to each of the electrodes in the capillary nanopipettes and configured to apply an alternating voltage to each of the electrodes thereby producing positive and negative currents, and the current detecting circuit configured to detect both of the positive and negative currents,
wherein upon binding of an analyte in the sample to the tip of a capillary nanopipette both the positive and negative currents from the capillary nanopipette detected by the current detecting circuit are reduced.