	US 11,940,382 B2
	Assay with amplification
Stephen Y. Chou, Princeton, NJ (US); Wei Ding, East Windsor, NJ (US); Yufan Zhang, Monmouth Junction, NJ (US); Ji Qi, Hillsborough, NJ (US); and Ji Li, Princeton, NJ (US)
Assigned to Essenlix Corporation, Monmouth Junction, NJ (US)
Appl. No. 16/484,803
Filed by Essenlix Corporation, Monmouth Junction, NJ (US)
PCT Filed Feb. 8, 2018, PCT No. PCT/US2018/017492 § 371(c)(1), (2) Date Aug. 8, 2019, PCT Pub. No. WO2018/148461, PCT Pub. Date Aug. 16, 2018.
Claims priority of provisional application 62/459,303, filed on Feb. 15, 2017.
Claims priority of provisional application 62/459,267, filed on Feb. 15, 2017.
Claims priority of provisional application 62/460,083, filed on Feb. 16, 2017.
Claims priority of provisional application 62/460,052, filed on Feb. 16, 2017.
Claims priority of provisional application 62/456,904, filed on Feb. 9, 2017.
Claims priority of provisional application 62/457,075, filed on Feb. 9, 2017.
Claims priority of provisional application 62/457,084, filed on Feb. 9, 2017.
Prior Publication US 2020/0225161 A1, Jul. 16, 2020
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 21/65 (2006.01); G01N 21/64 (2006.01); G01N 21/66 (2006.01); G01N 33/543 (2006.01); G01N 33/58 (2006.01)

CPC G01N 21/65 (2013.01) [G01N 21/64 (2013.01); G01N 21/66 (2013.01); G01N 33/54366 (2013.01); G01N 33/582 (2013.01); G01N 2201/06113 (2013.01)]

89 Claims

1. A device for analyzing a sample comprising:

a first plate, a second plate, a surface amplification layer, and a capture agent, wherein:

(a) the first and second plates are movable relative to each other into different configurations including an open configuration and a closed configuration, and have, on their respective surface, a sample contact area for contacting the sample that contains a target analyte,

(b) the surface amplification layer is on one of the sample contact areas,

(c) the capture agent is immobilized on the surface amplification layer, wherein the capture agent specifically binds the target analyte,

wherein the surface amplification layer amplifies an optical signal from the target analyte or a label attached to the target analyte in a proximity-dependent manner that makes the target analyte or the label attached to the target analyte closer to the surface amplification layer having a larger optical signal than that of the target analyte or the label attached to the target analyte farther away from the surface amplification layer,

wherein in the open configuration, the average spacing between the inner surfaces of the two plates is at least 200 μm; and

wherein in the closed configuration, at least part of the sample is between the two plates, and the average spacing between the inner surfaces of the first and second plates is less than 200 μm.