CPC G01N 21/3563 (2013.01) [C12Q 1/02 (2013.01); G01J 5/22 (2013.01); G01N 21/171 (2013.01); G01J 2005/0077 (2013.01); G01N 2021/1712 (2013.01); G01N 2201/0612 (2013.01); G01N 2201/0697 (2013.01); G02B 21/02 (2013.01); G02B 21/06 (2013.01); G02B 21/18 (2013.01); H01S 5/3401 (2013.01)] | 14 Claims |
1. A device for microscopic analysis of a sample, comprising:
a) a mid-IR optical source that generates an infrared beam;
b) an optical source that generates a probe beam;
c) beam combining optics configured to combine the infrared beam and the probe beam as combined beams;
d) an objective configured to focus the combined beams on to the sample;
e) a detector to detect probe light from at least one of: the probe beam transmitted through the sample and the probe beam returning from the sample; and
f) a data acquisition and processing system configured to acquire and process the detected probe light from the detector to generate a signal indicative of IR absorption by the sample, wherein the signal indicative of IR absorption has a spatial resolution of less than 1 micrometer,
wherein the data acquisition and processing system is configured to obtain measurements at different depths within the sample and generate a plurality of signals indicative of IR absorption each at the different depths for use in generating a set of depth-resolved maps of IR absorption of the sample.
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