US 11,940,358 B2
Eosin staining techniques
Benjamin H. Blehm, Tucson, AZ (US); Evan T. Graves, Tucson, AZ (US); and Edward E. Durrant, Tucson, AZ (US)
Assigned to Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed by Ventana Medical Systems, Inc., Tucson, AZ (US)
Filed on Jun. 3, 2020, as Appl. No. 16/891,896.
Application 16/891,896 is a continuation of application No. PCT/EP2018/086062, filed on Dec. 20, 2018.
Claims priority of provisional application 62/610,211, filed on Dec. 24, 2017.
Prior Publication US 2020/0340892 A1, Oct. 29, 2020
Int. Cl. G01N 1/30 (2006.01)
CPC G01N 1/30 (2013.01) [G01N 2001/302 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of staining a specimen disposed on a substrate with an automated specimen processing apparatus, the method comprising:
(a) dispensing a predetermined amount of a hematoxylin solution to the specimen;
(b) at least partially removing the hematoxylin solution from the specimen;
(c) dispensing a predetermined amount of an alkaline solution to the specimen;
(d) at least partially removing the alkaline solution from the specimen;
(e) dispensing a predetermined amount of at least one additional solution to the specimen;
(f) at least partially removing the at least one additional solution from the specimen;
(g) dispensing a predetermined amount of an eosin staining solution to the specimen;
(h) at least partially removing the eosin solution from the specimen; and
(i) dispensing a predetermined amount of an acid solution to the specimen, wherein the predetermined amount of the acid solution is dispensed to the specimen following the dispensing and at least partial removal of the eosin solution from the specimen.