Methods for simultaneous amplification of target loci
Joshua Babiarz, Castro Valley, CA (US); Tudor Pompiliu Constantin, Berkeley, CA (US); Lane A. Eubank, San Carlos, CA (US); George Gemelos, Portland, OR (US); Matthew Micah Hill, Belmont, CA (US); Huseyin Eser Kirkizlar, Los Angeles, CA (US); Matthew Rabinowitz, San Francisco, CA (US); Onur Sakarya, Redwood City, CA (US); Styrmir Sigurjonsson, San Jose, CA (US); and Bernhard Zimmermann, Manteca, CA (US)
Assigned to Natera, Inc., San Carlos, CA (US)
Filed by Natera, Inc., San Carlos, CA (US)
Filed on Sep. 11, 2020, as Appl. No. 17/018,966.
Application 17/018,966 is a division of application No. 16/140,298, filed on Sep. 24, 2018.
Application 16/140,298 is a continuation of application No. 14/918,544, filed on Oct. 20, 2015, granted, now 10,316,362, issued on Jun. 11, 2019.
Application 14/918,544 is a continuation in part of application No. 14/877,925, filed on Oct. 7, 2015, abandoned.
Application 14/877,925 is a continuation in part of application No. 14/692,703, filed on Apr. 21, 2015, granted, now 10,179,937, issued on Jan. 15, 2019.
Application 14/692,703 is a continuation in part of application No. 14/538,982, filed on Nov. 24, 2014, granted, now 9,677,118, issued on Jun. 13, 2017.
Application 14/877,925 is a continuation in part of application No. 14/225,356, filed on Mar. 25, 2014, abandoned.
Application 14/225,356 is a continuation in part of application No. 13/780,022, filed on Feb. 28, 2013, abandoned.
Application 13/780,022 is a continuation of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 14/225,356 is a continuation of application No. PCT/US2012/058578, filed on Oct. 3, 2012.
Application 13/780,022 is a continuation in part of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 13/683,604 is a continuation in part of application No. PCT/US2012/058578, filed on Oct. 3, 2012.
Application PCT/US2012/058578 is a continuation in part of application No. 13/335,043, filed on Dec. 22, 2011, granted, now 10,113,196, issued on Oct. 30, 2018.
Application 13/335,043 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/300,235 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application 13/683,604 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/300,235 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application PCT/US2012/058578 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/335,043 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/300,235 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application 13/300,235 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Claims priority of provisional application 62/148,173, filed on Apr. 15, 2015.
Claims priority of provisional application 62/147,377, filed on Apr. 14, 2015.
Claims priority of provisional application 62/146,188, filed on Apr. 10, 2015.
Claims priority of provisional application 62/066,514, filed on Oct. 21, 2014.
Claims priority of provisional application 61/994,791, filed on May 16, 2014.
Claims priority of provisional application 61/987,407, filed on May 1, 2014.
Claims priority of provisional application 61/982,245, filed on Apr. 21, 2014.
Claims priority of provisional application 61/634,431, filed on Feb. 29, 2012.
Claims priority of provisional application 61/675,020, filed on Jul. 24, 2012.
Claims priority of provisional application 61/683,331, filed on Aug. 15, 2012.
Claims priority of provisional application 61/542,508, filed on Oct. 3, 2011.
Claims priority of provisional application 61/426,208, filed on Dec. 22, 2010.
Claims priority of provisional application 61/571,248, filed on Jun. 23, 2011.
Claims priority of provisional application 61/448,547, filed on Mar. 2, 2011.
Claims priority of provisional application 61/462,972, filed on Feb. 9, 2011.
Claims priority of provisional application 61/398,159, filed on Jun. 21, 2010.
Claims priority of provisional application 61/395,850, filed on May 18, 2010.
Prior Publication US 2020/0407798 A1, Dec. 31, 2020
1. A method of nested amplification, the method comprising:
(a) performing a first amplification on a plurality of target loci of a nucleic acid sample using a first library of primers comprising a plurality of target-specific primers and optionally a universal primer,
(b) performing a second, nested amplification on the target loci using a second library of primers comprising a plurality of inner target-specific primers and optionally a universal primer to produce amplified products,
wherein, in (a), (b), or both, the length of an annealing step is greater than 3 minutes, and at least two of the primers have a melting temperature lower than an annealing temperature of the annealing step.