US 11,939,630 B2
Fluorescent PCR method for detecting HLA-B*15:02 allele and specific primer probe combination thereof
Penggao Dai, Xi'an (CN); Zihua Zhong, Xi'an (CN); Hao Wang, Xi'an (CN); Zhiye Cai, Xi'an (CN); Lei Meng, Xi'an (CN); and Le Wang, Xi'an (CN)
Assigned to Shaanxi Lifegen Co., Ltd., Xi'an (CN)
Filed by Shaanxi Lifegen Co., Ltd., Xi'an (CN)
Filed on Aug. 19, 2021, as Appl. No. 17/406,375.
Claims priority of application No. 202011431450.8 (CN), filed on Dec. 7, 2020.
Prior Publication US 2022/0177955 A1, Jun. 9, 2022
Int. Cl. C12Q 1/6858 (2018.01); C12Q 1/6881 (2018.01)
CPC C12Q 1/6858 (2013.01) [C12Q 1/6881 (2013.01)] 7 Claims
 
1. A specific primer probe combination for HLA-B*15:02 allele, comprising:
HLA-B*15:02 allele-specific forward primer Fm, the nucleotide sequence of Fm is set forth in SEQ ID NO: 1;
non-HLA-B*15:02 allele-specific forward primer Fn1, the nucleotide sequence of Fn1 is set forth in SEQ ID NO: 2;
non-HLA-B*15:02 allele-specific forward primer Fn2, the nucleotide sequence of Fn2 is set forth in SEQ ID NO: 3;
reverse primer R, the nucleotide sequence of R is set forth in SEQ ID NO: 4; and
probe P: 5′-Carboxy-X-Rhodamine-ACGCAGCCTGGGACCCTGTGTGCCAGCA quenching group-3′, the nucleotide sequence between Carboxy-X-Rhodamine and the quenching group is set forth in SEQ ID NO: 5;
wherein Carboxy-X-Rhodamine is a fluorescent group, and the quenching group has a chemical structure of

OG Complex Work Unit Chemistry