CPC C12Q 1/6858 (2013.01) [C12Q 1/6881 (2013.01)] | 7 Claims |
1. A specific primer probe combination for HLA-B*15:02 allele, comprising:
HLA-B*15:02 allele-specific forward primer Fm, the nucleotide sequence of Fm is set forth in SEQ ID NO: 1;
non-HLA-B*15:02 allele-specific forward primer Fn1, the nucleotide sequence of Fn1 is set forth in SEQ ID NO: 2;
non-HLA-B*15:02 allele-specific forward primer Fn2, the nucleotide sequence of Fn2 is set forth in SEQ ID NO: 3;
reverse primer R, the nucleotide sequence of R is set forth in SEQ ID NO: 4; and
probe P: 5′-Carboxy-X-Rhodamine-ACGCAGCCTGGGACCCTGTGTGCCAGCA quenching group-3′, the nucleotide sequence between Carboxy-X-Rhodamine and the quenching group is set forth in SEQ ID NO: 5;
wherein Carboxy-X-Rhodamine is a fluorescent group, and the quenching group has a chemical structure of
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