CPC C12Q 1/6832 (2013.01) [C12Q 1/6816 (2013.01); C12Q 1/6823 (2013.01); C12Q 1/686 (2013.01)] | 14 Claims |
1. A method that detects a nucleic-acid target molecule, the method comprising:
in a first stage, introducing, to recognition probes,
a sample solution containing the nucleic-acid target molecule, and
recognition restriction endonucleases that cleave a target/recognition-probe duplex to produce a first nucleic-acid product;
in a second stage, introducing, to amplification probes of two types, each bound to a solid support and each including a marker component, including a first-type amplification probe and a second-type amplification probe,
the first nucleic-acid product, and
two types of restriction endonuclease, including a second-type restriction endonuclease that cleaves a first-product/first-type-amplification-probe duplex and a third-product/first-type-amplification-probe duplex to produce a second nucleic-acid product and a third-type restriction endonuclease that cleaves a second-product/second-type-amplification-probe duplex to produce the third nucleic-acid product; and
detecting marker component no longer bound to a solid support produced by multiple cycles of product production in the second stage.
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