CPC C12Q 1/6827 (2013.01) [C12N 9/0071 (2013.01); C12N 9/78 (2013.01); C12Q 1/6806 (2013.01); C12Y 114/11 (2013.01); C12Y 305/04005 (2013.01)] | 4 Claims |
1. A method, comprising:
(a) reacting 5-hydroxymethylcytosine (5hmC) in a nucleic acid sample with a glucosyl transferase in the absence of a Tet enzyme to form a nucleic acid product with a modified hmC;
(b) converting cytosine (C) to uracil and 5-methyl cytosine (5mC) to thymidine in the nucleic acid product from (a) with a protein that has at least 90% sequence homology to apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (Apobec);
(c) optionally amplifying the reaction product from (b); and
(d) hybridizing and/or sequencing the product from (b) or amplicon thereof.
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