US 11,939,627 B2
Methods and devices for single-molecule whole genome analysis
Ming H. Xiao, Huntingdon Valley, PA (US); and Han Cao, San Diego, CA (US)
Assigned to Bionano Genomics, Inc., San Diego, CA (US)
Filed by Bionano Genomics, Inc., San Diego, CA (US)
Filed on Mar. 23, 2021, as Appl. No. 17/210,298.
Application 17/210,298 is a continuation of application No. 16/593,915, filed on Oct. 4, 2019, granted, now 10,995,364.
Application 16/593,915 is a continuation of application No. 15/381,787, filed on Dec. 16, 2016, granted, now 10,435,739, issued on Oct. 8, 2019.
Application 15/381,787 is a continuation of application No. 13/765,353, filed on Feb. 12, 2013, granted, now 9,536,041, issued on Jan. 3, 2017.
Application 13/765,353 is a continuation of application No. 13/001,697, granted, now 8,628,919, issued on Jan. 14, 2014, previously published as PCT/US2009/049244, filed on Jun. 30, 2009.
Claims priority of provisional application 61/076,785, filed on Jun. 30, 2008.
Prior Publication US 2022/0017947 A1, Jan. 20, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/6818 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6869 (2018.01); G16B 20/00 (2019.01); G16B 20/10 (2019.01); G16B 20/20 (2019.01); G16B 20/30 (2019.01)
CPC C12Q 1/6818 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/6825 (2013.01); C12Q 1/6869 (2013.01); G16B 20/00 (2019.02); G16B 20/10 (2019.02); G16B 20/20 (2019.02); G16B 20/30 (2019.02)] 20 Claims
 
1. A method of characterizing a first DNA and a second DNA, comprising:
labeling a plurality of sequence-specific locations on a first DNA and a second DNA with a labeling technique that does not cut or nick a strand of DNA;
linearizing at least a portion of the labeled first DNA and a portion of the labeled second DNA;
detecting a recognizable pattern of labels on the linearized first DNA and on the linearized second DNA, wherein the labels are indicative of a sequence characteristic of the first DNA and a sequence characteristic of the second DNA; and
comparing the recognizable patterns of the first and second DNAs to ascertain a similarity or difference in a sequence characteristic of the first and second DNAs.