US 11,939,624 B2
Method for labeling ligation products with cell-specific barcodes II
Garry P. Nolan, Redwood City, CA (US)
Assigned to ROCHE SEQUENCING SOLUTIONS, INC., Pleasanton, CA (US)
Filed by Roche Sequencing Solutions, Inc., Pleasanton, CA (US)
Filed on Oct. 12, 2023, as Appl. No. 18/379,589.
Application 18/379,589 is a continuation of application No. 18/233,221, filed on Aug. 11, 2023.
Application 18/233,221 is a continuation of application No. 18/134,003, filed on Apr. 12, 2023, granted, now 11,781,171, issued on Oct. 10, 2023.
Application 18/134,003 is a continuation of application No. 18/086,369, filed on Dec. 21, 2022, granted, now 11,708,599, issued on Jul. 25, 2023.
Application 18/086,369 is a continuation of application No. 17/951,013, filed on Sep. 22, 2022, granted, now 11,667,956, issued on Jun. 6, 2023.
Application 17/951,013 is a continuation of application No. 17/870,697, filed on Jul. 21, 2022, granted, now 11,566,278, issued on Jan. 31, 2023.
Application 17/870,697 is a continuation of application No. 16/795,203, filed on Feb. 19, 2020, abandoned.
Application 16/795,203 is a continuation of application No. 16/147,250, filed on Sep. 28, 2018, granted, now 10,982,256, issued on Apr. 20, 2021.
Application 16/147,250 is a continuation of application No. 13/981,711, granted, now 10,144,950, issued on Dec. 4, 2018, previously published as PCT/US2012/023411, filed on Jan. 31, 2012.
Claims priority of provisional application 61/444,067, filed on Feb. 17, 2011.
Claims priority of provisional application 61/437,854, filed on Jan. 31, 2011.
Prior Publication US 2024/0068013 A1, Feb. 29, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6816 (2018.01); C12Q 1/686 (2018.01)
CPC C12Q 1/6806 (2013.01) [C12Q 1/6816 (2013.01); C12Q 1/686 (2013.01)] 26 Claims
OG exemplary drawing
 
1. A method for barcoding, comprising:
performing a ligation assay on target nucleic acid molecules that are in or on cells or cell organelles to produce ligation products; and
adding cell-origination barcodes onto the ligation products or complements thereof by a split-pool barcoding process.