	US 11,939,619 B2
	Glucoamylase engineered yeast and fermentation methods
Chris Miller, Andover, MN (US); Brian Rush, Minneapolis, MN (US); Joshua Dunn, Boston, MA (US); and Brynne Stanton, Boston, MA (US)
Assigned to CARGILL, INCORPORATED, Wayzata, MN (US)
Filed by CARGILL, INCORPORATED, Wayzata, MN (US)
Filed on Mar. 30, 2022, as Appl. No. 17/657,241.
Application 17/657,241 is a continuation of application No. 16/976,550, granted, now 11,306,330, previously published as PCT/US2019/019805, filed on Feb. 27, 2019.
Claims priority of provisional application 62/636,716, filed on Feb. 28, 2018.
Prior Publication US 2022/0228175 A1, Jul. 21, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12P 7/10 (2006.01); C12N 1/18 (2006.01); C12N 9/34 (2006.01); C12R 1/865 (2006.01)

CPC C12P 7/10 (2013.01) [C12N 9/2428 (2013.01); C12N 1/185 (2021.05); C12P 2203/00 (2013.01); C12R 2001/865 (2021.05); C12Y 302/01003 (2013.01)]

18 Claims

1. An engineered yeast comprising an exogenous nucleic acid encoding a glucoamylase comprising a sequence having 97% or greater sequence identity to SEQ ID NO:4, wherein the yeast is capable of producing ethanol at a rate of 1 g/L*h or greater during a fermentation process.