US 11,939,614 B2
Genes and proteins for aromatic polyketide synthesis
Jonathan E. Page, Vancouver (CA); and Steve Gagne, Aberdeen (CA)
Assigned to National Research Council of Canada, Ottawa (CA); and University of Saskatchewan, Saskatoon (CA)
Filed by National Research Council of Canada, Ottawa (CA); and University of Saskatchewan, Saskatoon (CA)
Filed on Mar. 10, 2022, as Appl. No. 17/691,933.
Application 16/917,362 is a division of application No. 16/051,701, filed on Aug. 1, 2018, granted, now 10,718,000, issued on Jul. 21, 2020.
Application 17/691,933 is a continuation of application No. 16/917,362, filed on Jun. 30, 2020, granted, now 11,306,335.
Application 16/051,701 is a continuation of application No. 15/438,291, filed on Feb. 21, 2017, granted, now 10,059,971, issued on Aug. 20, 2018.
Application 15/438,291 is a continuation of application No. 13/641,272, granted, now 9,611,460, issued on Apr. 4, 2017, previously published as PCT/CA2011/000428, filed on Apr. 15, 2011.
Claims priority of provisional application 61/324,343, filed on Apr. 15, 2010.
Prior Publication US 2022/0195473 A1, Jun. 23, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/82 (2006.01); C12N 15/52 (2006.01); C12N 15/70 (2006.01); C12P 7/22 (2006.01); C12P 17/06 (2006.01)
CPC C12P 17/06 (2013.01) [C12N 15/52 (2013.01); C12N 15/70 (2013.01); C12P 7/22 (2013.01); C12Y 404/01026 (2015.07)] 13 Claims
 
1. A host cell transformed with: i) a nucleotide sequence as set forth in SEQ ID NO: 1 or 3 or a codon degenerate nucleotide sequence thereof; ii) a nucleotide sequence of 303 nucleotides that encodes a polypeptide with an amino acid sequence of SEQ ID NO: 2 or a conservatively substituted amino acid sequence thereof; or iii) a fragment of i) or ii) having a length of at least 300 base pairs; wherein the nucleic acid molecule encodes a polypeptide with polyketide cyclase activity.