	US 11,939,613 B2
	Production of cannabinoids in yeast
Maxim Mikheev, Fremont, CA (US); and Difeng Gao, Union City, CA (US)
Assigned to BIOMEDICAN, INC., Fremont, CA (US)
Filed by BIOMEDICAN, INC., Fremont, CA (US)
Filed on Sep. 14, 2021, as Appl. No. 17/474,708.
Application 17/474,708 is a division of application No. 16/630,051, granted, now 11,149,291, previously published as PCT/US2018/041890, filed on Jul. 12, 2018.
Claims priority of provisional application 62/531,827, filed on Jul. 12, 2017.
Prior Publication US 2021/0403965 A1, Dec. 30, 2021
Int. Cl. C12N 9/00 (2006.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12P 7/22 (2006.01); C12P 17/06 (2006.01)

CPC C12P 17/06 (2013.01) [C12N 9/0004 (2013.01); C12N 9/0006 (2013.01); C12N 9/1029 (2013.01); C12N 9/1085 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12Y 101/01034 (2013.01); C12Y 121/03007 (2015.07); C12Y 121/03008 (2015.07); C12Y 203/0102 (2013.01); C12Y 203/01206 (2015.07); C12Y 404/01026 (2015.07); C12Y 604/01002 (2013.01)]

10 Claims

1. A genetically modified yeast comprising:

(a) a first polynucleotide encoding a first polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:4, wherein said first polynucleotide has farnesyl diphosphate synthase (ERG20) activity;

(b) a second polynucleotide encoding a second polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:7, wherein said second polypeptide has olivetol synthase (OLS) activity;

(c) a third polynucleotide encoding a third polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:8, wherein said third polypeptide has olivetolic acid cyclase (OAC) activity;

(d) a fourth polynucleotide encoding a fourth polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:12, wherein said fourth polypeptide has soluble aromatic prenyltransferase (NphB) activity; and

(e) at least 5% dry weight of fatty acids or fats.