	US 11,939,611 B2
	Biocatalytic processes and materials for enhanced carbon utilization
Gary Smith, Wilton (GB); Alex Van Eck Conradie, Eaglescliffe (GB); Gregory S. Kirby, Avondale, PA (US); and Paul S. Pearlman, Thornton, PA (US)
Assigned to INV NYLON CHEMICALS AMERICAS, LLC, Wilmington, DE (US)
Filed by INV Nylon Chemicals Americas, LLC, Wilmington, DE (US)
Filed on Feb. 17, 2017, as Appl. No. 15/436,315.
Claims priority of provisional application 62/297,626, filed on Feb. 19, 2016.
Prior Publication US 2017/0240936 A1, Aug. 24, 2017
Int. Cl. C12P 13/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12N 1/20 (2006.01); C12P 5/00 (2006.01); C12P 5/02 (2006.01); C12P 7/06 (2006.01); C12P 7/24 (2006.01); C12P 7/625 (2022.01); C25B 1/04 (2021.01); F01D 15/10 (2006.01); F01K 7/16 (2006.01); F01K 11/02 (2006.01)

CPC C12P 13/001 (2013.01) [C12M 29/00 (2013.01); C12M 41/00 (2013.01); C12N 1/20 (2013.01); C12P 5/00 (2013.01); C12P 5/007 (2013.01); C12P 5/02 (2013.01); C12P 7/065 (2013.01); C12P 7/24 (2013.01); C12P 7/625 (2013.01); C12P 13/00 (2013.01); C25B 1/04 (2013.01); F01D 15/10 (2013.01); F01K 7/16 (2013.01); F01K 11/02 (2013.01); F05D 2220/31 (2013.01); Y02E 50/10 (2013.01); Y02E 50/30 (2013.01); Y02E 60/36 (2013.01)]

14 Claims

1. A biocatalytic process comprising:

providing an aqueous thin stillage stream from a bioethanol production process to a fermentation zone, wherein the aqueous thin stillage stream comprises glycerol and lactic acid;

providing a gaseous stream to the fermentation zone, wherein the gaseous stream comprises at least one substrate selected from CO₂, H₂, CO₂/H₂, methane, and CO, wherein the aqueous thin stillage stream and the gaseous stream are contacted and/or mixed in the fermentation zone; and

providing a Cupriavidus necator to the fermentation zone, wherein the Cupriavidus necator mixotrophically metabolizes glycerol and lactic acid in the aqueous thin stillage stream and the at least one substrate in the gaseous stream to produce a product, wherein said product produced is at least 2.1× up to 4.2× more than fermentation in Escherichia coli;

wherein the product comprises 7-aminoheptanoic acid.