	US 11,939,589 B2
	Methods for genetic control of insect infestations in plants and compositions thereof
James A. Baum, Webster Groves, MO (US); Claire A. CaJacob, Chesterfield, MO (US); Pascale Feldmann, Ghent (BE); Gregory R. Heck, Crystal Lake Park, MO (US); Irene Maria Antonia Nooren, XH Utrecht (NL); Geert Plaetinck, Merelbeke-Bottelare (BE); Wendy T. Maddelein, Gijzenzele (BE); and Ty T. Vaughn, Clayton, MO (US)
Assigned to Monsanto Technology LLC, St. Louis, MO (US)
Filed by Monsanto Technology LLC, St. Louis, MO (US)
Filed on Apr. 26, 2022, as Appl. No. 17/729,892.
Application 17/729,892 is a division of application No. 16/706,285, filed on Dec. 6, 2019, granted, now 11,312,975.
Application 16/706,285 is a division of application No. 15/610,220, filed on May 31, 2017, granted, now 10,538,783, issued on Jan. 21, 2020.
Application 15/610,220 is a division of application No. 15/348,706, filed on Nov. 10, 2016, abandoned.
Application 15/348,706 is a division of application No. 13/855,328, filed on Apr. 2, 2013, granted, now 9,695,439, issued on Jul. 4, 2017.
Application 13/855,328 is a division of application No. 12/973,783, filed on Dec. 20, 2010, granted, now 8,759,611, issued on Jun. 24, 2014.
Application 12/973,783 is a division of application No. 11/522,307, filed on Sep. 15, 2006, granted, now 7,943,819, issued on May 17, 2011.
Claims priority of provisional application 60/718,034, filed on Sep. 16, 2005.
Prior Publication US 2022/0315945 A1, Oct. 6, 2022
Int. Cl. C12N 15/82 (2006.01); C07H 21/04 (2006.01); C07K 14/435 (2006.01); C12N 15/113 (2010.01)

CPC C12N 15/8286 (2013.01) [C07H 21/04 (2013.01); C07K 14/43536 (2013.01); C07K 14/43563 (2013.01); C12N 15/113 (2013.01); C12N 15/8218 (2013.01); C12N 15/8282 (2013.01); C12N 15/8285 (2013.01); Y02A 40/146 (2018.01)]

6 Claims

1. A method for improving the yield of a crop produced from a crop plant subjected to insect pest infestation, said method comprising the steps of,

a) introducing a recombinant polynucleotide into said crop plant, wherein the recombinant polynucleotide comprises a first polynucleotide sequence selected from the group consisting of:

(i) a polynucleotide comprising the nucleic acid sequence of SEQ ID NO: 818, 822, 823 or 824,

(ii) a polynucleotide comprising at least 95% sequence identity to the nucleic acid sequence of SEQ ID NO: 818, 822, 823 or 824,

(iii) a polynucleotide comprising a fragment of at least 21 contiguous nucleotides of a nucleic acid sequence of SEQ ID NO: 818, 822, 823 or 824, wherein ingestion by a coleopteran plant pest of a double stranded ribonucleotide sequence comprising at least one strand that is complementary to said fragment inhibits the growth of said pest, and

(iv) a polynucleotide comprising a complement of the sequence of (i), (ii), or (iii), wherein the polynucleotide is operably linked to a heterologous promoter, and wherein the recombinant nucleic acid molecule comprises a second polynucleotide that is the complement of the first polynucleotide; and

b) cultivating the crop plant to allow the expression of said polynucleotide, wherein expression of the polynucleotide inhibits feeding by insects pests and loss of yield due to pest infestation.