US 11,939,587 B2
Plants having increased tolerance to herbicides
Doreen Schachtschabel, Ludwigshafen (DE); Eva Hollenbach, Limburgerhof (DE); Mihiret Tekeste Sisay, Ludwigshafen (DE); Thomas Zierke, Boehl-Iggelheim (DE); and Danny Geerdink, Ludwigshafen (DE)
Assigned to BASF SE, Ludwigshafen (DE)
Appl. No. 16/962,562
Filed by BASF SE, Ludwigshafen (DE)
PCT Filed Jan. 15, 2019, PCT No. PCT/IB2019/050310
§ 371(c)(1), (2) Date Jul. 16, 2020,
PCT Pub. No. WO2019/142099, PCT Pub. Date Jul. 25, 2019.
Claims priority of application No. 18152064 (EP), filed on Jan. 17, 2018.
Prior Publication US 2020/0399651 A1, Dec. 24, 2020
Int. Cl. C12N 15/00 (2006.01); A01H 1/00 (2006.01); C12N 9/78 (2006.01); C12N 15/82 (2006.01)
CPC C12N 15/8274 (2013.01) [A01H 1/00 (2013.01); C12N 9/78 (2013.01); C12N 15/00 (2013.01); C12Y 305/04 (2013.01)] 5 Claims
 
1. An isolated, recombinantly produced and/or synthetic nucleic acid molecule comprising a nucleic acid molecule encoding a mutated melamine deaminase (TriA) polypeptide having at least 90% sequence identity with the amino acid sequence of the TriA polypeptide sequence SEQ ID NO: 2, wherein the amino acid sequence of the mutated TriA polypeptide comprises substitutions at positions corresponding to positions 92, 93, 155, and 157 of SEQ ID NO: 2, wherein the substitutions for each corresponding position are selected from the following:
L92C, L92D, L92E, L92G, L92N, L92Q, L925, L92T, L92V, or L92A;
Y93L, Y93A, Y93C, Y93E, Y93F, Y93G, Y93H, Y93I, Y93K, Y93M, Y93N, Y93Q, Y93R, Y93S, Y93T, Y93V, or Y93W;
M155T, M155A, M155C, M155H, M155I, M155K, M155L, M155N, M155P, M155Q, M155S, or M155V; and
F157L, F157A, F157C, F157G, F157H, F157I, F157K, F157M, F157N, F157Q, F157R, F157S, F157T, F157V, F157W, or F157Y; and
wherein expression of the nucleic acid molecule in a plant cell, a plant, or a part thereof confers increased herbicide tolerance or resistance to triazine herbicides, as compared to a corresponding, non-transformed, wild type plant cell, plant, or part thereof.