US 11,939,586 B2
Means and methods for improved regeneration
Lieven De Veylder, Drongen (BE); and Jefri Heyman, Roosdaal (BE)
Assigned to VIB VZW, Ghent (BE); and Universiteit Gent, Ghent (BE)
Appl. No. 16/340,949
Filed by VIB VZW, Ghent (BE); and UNIVERSITEIT GENT, Ghent (BE)
PCT Filed Oct. 11, 2017, PCT No. PCT/EP2017/075937
§ 371(c)(1), (2) Date Apr. 10, 2019,
PCT Pub. No. WO2018/069392, PCT Pub. Date Apr. 19, 2018.
Claims priority of application No. 16193308 (EP), filed on Oct. 11, 2016.
Prior Publication US 2019/0225976 A1, Jul. 25, 2019
Int. Cl. C12N 15/82 (2006.01); C07K 14/415 (2006.01); C12N 9/00 (2006.01); C12N 15/113 (2010.01)
CPC C12N 15/8241 (2013.01) [C07K 14/415 (2013.01); C12N 9/00 (2013.01); C12N 15/113 (2013.01); C12N 2800/24 (2013.01); C12N 2800/30 (2013.01); C12N 2820/65 (2013.01); C12N 2830/50 (2013.01); C12N 2830/65 (2013.01)] 16 Claims
 
1. A composition comprising:
a first chimeric gene construct and a second chimeric gene construct;
the first chimeric gene construct comprising the following operably linked DNA elements:
a) a plant expressible promoter,
b) a DNA region encoding an Ethylene Response Factor (ERF) comprising an SCL/PAT1 interaction motif, and
c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant, and
the second chimeric gene construct comprising the following operably linked DNA elements:
a) a plant expressible promoter,
b) a DNA region encoding Phytochrome A Signal Transduction 1 (PAT1), and
c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant
wherein expression of the first chimeric gene construct and the second chimeric gene construct in a plant leads to the formation of an ERF/PAT1 complex,
wherein the ERF comprising an SCL/PAT1 interaction motif is ERF115, and
wherein the plant has increased plant cell regeneration potential without additional hormones as compared to an otherwise identical plant lacking the first chimeric gene construct and the second chimeric gene construct.