CPC C12N 15/8206 (2013.01) [C12N 5/10 (2013.01); C12N 15/85 (2013.01); C12N 15/87 (2013.01); A61K 48/00 (2013.01)] | 16 Claims |
1. A method of transfecting mammalian cells with a RNA, comprising:
disposing the mammalian cells with the RNA in a transfection medium, wherein the mammalian cells are immune competent cells
applying 2, 3 or 4 pulses to the mammalian cells at a field strength of 1,200 V/cm and a time constant from about 0.5 msec to about 5 msec to produce transfected mammalian cells,
wherein the transfected mammalian cells have a viability of at least 70%.
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