US 11,939,584 B2
Methods and microorganisms for making 2,3-butanediol and derivatives thereof from C1 carbons
Xinhua Zhao, Dublin, CA (US); Mark Anton Held, Emeryville, CA (US); Tina Huynh, Oakland, CA (US); Lily Yuin Chao, San Francisco, CA (US); Na Trinh, Walnut Creek, CA (US); Matthias Helmut Schmalisch, South San Francisco, CA (US); Bryan Yeh, Walnut Creek, CA (US); James Kealey, Sebastopol, CA (US); and Kevin Lee Dietzel, Pacifica, CA (US)
Assigned to PRECIGEN, INC., Germantown, MD (US)
Filed by PRECIGEN, INC., Germantown, MD (US)
Filed on Jul. 31, 2021, as Appl. No. 17/444,188.
Application 17/444,188 is a division of application No. 16/481,799, granted, now 11,111,496, previously published as PCT/US2018/015909, filed on Jan. 30, 2018.
Claims priority of provisional application 62/588,985, filed on Nov. 21, 2017.
Claims priority of provisional application 62/512,312, filed on May 30, 2017.
Claims priority of provisional application 62/504,626, filed on May 11, 2017.
Claims priority of provisional application 62/451,819, filed on Jan. 30, 2017.
Prior Publication US 2022/0049259 A1, Feb. 17, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 15/52 (2006.01); C12N 1/20 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 15/74 (2006.01); C12P 5/02 (2006.01); C12P 7/16 (2006.01); C12P 7/18 (2006.01)
CPC C12N 15/52 (2013.01) [C12N 1/20 (2013.01); C12N 9/0006 (2013.01); C12N 9/1022 (2013.01); C12N 9/88 (2013.01); C12N 15/74 (2013.01); C12P 5/023 (2013.01); C12P 7/16 (2013.01); C12P 7/18 (2013.01); C12Y 101/01304 (2013.01); C12Y 202/01006 (2013.01); C12Y 401/01004 (2013.01); C12Y 401/01005 (2013.01)] 15 Claims
 
1. A vector comprising two or more genes encoding: (a) an acetoin reductase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 9; (b) an alpha-acetolactate decarboxylase comprising an amino acid sequence that is at least 90% identical to SEQ ID NO: 7; and/or (c) an acetolactate synthase comprising an amino acid sequence that is at least 90% identical to any one of SEQ ID NO: 1, 3, and 19.