US 11,939,583 B2
Method of producing autotrophic organisms with altered photorespiration and improved CO2 fixation
Lennart Schada Von Borzyskowski, Marburg (DE); Tobias Jürgen Erb, Marburg (DE); and Jan Zarzycki, Marburg (DE)
Assigned to Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Munich (DE)
Appl. No. 17/047,974
Filed by Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V., Munich (DE)
PCT Filed Apr. 15, 2019, PCT No. PCT/EP2019/059715
§ 371(c)(1), (2) Date Oct. 15, 2020,
PCT Pub. No. WO2019/201880, PCT Pub. Date Oct. 24, 2019.
Claims priority of application No. 18167406 (EP), filed on Apr. 15, 2018.
Prior Publication US 2021/0163956 A1, Jun. 3, 2021
Int. Cl. C12N 15/52 (2006.01); C12N 1/21 (2006.01); C12N 9/04 (2006.01); C12N 9/06 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 9/88 (2006.01); C12N 15/74 (2006.01)
CPC C12N 15/52 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0022 (2013.01); C12N 9/1096 (2013.01); C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12N 15/74 (2013.01); C12Y 101/01026 (2013.01); C12Y 104/03016 (2013.01); C12Y 206/01001 (2013.01); C12Y 301/03018 (2013.01); C12Y 401/03014 (2013.01); C12Y 403/0102 (2013.01)] 16 Claims
 
1. A method for the production of autotrophic microorganisms with altered photorespiration comprising introducing into an autotrophic microorganism expressing (i) phosphoglycolate phosphatase activity one or more nucleic acids encoding polypeptides having the enzymatic activities of:
(ii) glyoxylate reductase,
(iii) erythro-β-hydroxyaspartate aldolase belonging to the EC class 4.1.3.14,
(iv) erythro-β-hydroxyaspartate dehydratase belonging to the EC class 4.3.1.20,
(v) iminosuccinate reductase and
(vi) aspartate-glyoxylate transaminase, wherein the introduction of the one or more nucleic acids results in an altered autotrophic microorgansim having de novo expression of polypeptides having the enzymatic activities of
(ii) glyoxylate reductase,
(iii) erythro-β-hydroxyaspartate aldolase belonging to the EC class 4.1.3.14,
(iv) erythro-β-hydroxyaspartate dehydratase belonging to the EC class 4.3.1.20,
(v) iminosuccinate reductase, and
(vi) aspartate-glyoxylate transaminase.