CPC C12N 15/113 (2013.01) [A61K 9/0019 (2013.01); A61P 21/00 (2018.01); C07H 21/02 (2013.01); C12N 7/00 (2013.01); A61K 48/00 (2013.01); C12N 2310/141 (2013.01); C12N 2320/32 (2013.01); C12N 2330/51 (2013.01); C12N 2710/10043 (2013.01); C12Q 1/70 (2013.01)] | 19 Claims |
1. A method of producing a modified U6 promoter, the method comprising transfecting a host cell with a nucleic acid comprising:
a modified U6 promoter sequence comprising the nucleotide sequence set forth in SEQ ID NO: 4,
a mature guide strand of a miRNA comprising at least one detargeting sequence, wherein the detargeting sequence is a binding site for miRNA-122 and/or miRNA-208, and
5 or 6 thymidines at the 5′ end.
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