US 11,939,560 B2
Microfluidic cell culture systems
Margaret Haiganouch Magdesian, Quebec (CA); and Peter Thostrup, Viby J (DK)
Assigned to 9493662 Canada Inc., Montreal (CA)
Filed by 9493662 Canada Inc., Quebec (CA)
Filed on Mar. 10, 2022, as Appl. No. 17/691,599.
Application 17/691,599 is a continuation of application No. 14/909,061, granted, now 11,293,001, previously published as PCT/CA2014/000589, filed on Jul. 29, 2014.
Claims priority of provisional application 62/013,746, filed on Jun. 18, 2014.
Claims priority of provisional application 61/859,318, filed on Jul. 29, 2013.
Prior Publication US 2022/0195365 A1, Jun. 23, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 3/06 (2006.01); B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12M 1/12 (2006.01); C12M 1/34 (2006.01)
CPC C12M 23/16 (2013.01) [B01L 3/50255 (2013.01); B01L 3/502715 (2013.01); C12M 23/34 (2013.01); C12M 25/06 (2013.01); C12M 41/46 (2013.01); B01L 2200/0642 (2013.01); B01L 2200/0647 (2013.01)] 12 Claims
OG exemplary drawing
 
1. A microfluidic device comprising:
first and second compartments fluidly connected by microchannels extending therebetween, the first compartment comprising:
a main chamber having an upper wall and opposite sidewalls;
a first auxiliary room having a first peripheral wall joined to the upper wall of the main chamber and to a respective one of the opposite sidewalls, the first peripheral wall defining a first opening connecting the first auxiliary room to the main chamber;
a second auxiliary room having a second peripheral wall, the second auxiliary room being located opposite the first auxiliary room with the main chamber extending therebetween;
wherein the upper wall of the main chamber and the respective one of the opposite sidewalls extend from the first peripheral wall to define a side portion of the main chamber that tapers outwardly away from the first opening and toward a center portion of the upper wall of the main chamber;
the microchannels comprising inlets opened to the main chamber and aligned along a microchannel inlet plane, and being distributed in a side-by-side relationship relative to the opposite sidewalls; and
at least one of the opposite sidewalls intersecting with the microchannel inlet plane along an intersection line to form an angle ranging from 70° to 110° therewith.