CPC C11B 13/00 (2013.01) [C11B 3/003 (2013.01); C12N 9/2417 (2013.01); C12N 9/2428 (2013.01); C12N 9/50 (2013.01); C12N 9/52 (2013.01); C12N 9/58 (2013.01); C12P 7/10 (2013.01); C12P 7/64 (2013.01); B01D 3/002 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 304/00 (2013.01); Y02E 50/10 (2013.01)] | 24 Claims |
1. A process of recovering oil, comprising:
(a) liquefying a starch-containing material into dextrins with a bacterial alpha-amylase at a temperature above the initial gelatinization temperature, wherein the starch-containing material comprises corn;
(b) saccharifying the dextrins using a glucoamylase to form a sugar;
(c) fermenting the sugar in a fermentation medium into ethanol using a yeast fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup; and
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease having at least 60% sequence identity to the amino acid sequence of SEQ ID NO: 4 is added during step (a).
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