US 11,939,552 B2
Process of recovering oil
Nathaniel Edward Kreel, Louisburg, NC (US); and Joseph Mark Jump, Raleigh, NC (US)
Assigned to NOVOZYMES A/S, Bagsvaerd (DK)
Filed by Novozymes A/S, Bagsvaerd (DK)
Filed on Sep. 22, 2022, as Appl. No. 17/934,465.
Application 17/934,465 is a continuation of application No. 17/147,623, filed on Jan. 13, 2021, granted, now 11,505,765.
Application 17/147,623 is a continuation of application No. 16/449,703, filed on Jun. 24, 2019, granted, now 10,920,172, issued on Feb. 16, 2021.
Application 16/449,703 is a continuation of application No. 15/956,274, filed on Apr. 18, 2018, granted, now 10,731,104, issued on Aug. 4, 2020.
Application 15/956,274 is a continuation of application No. 14/901,504, granted, now 10,035,973, issued on Jul. 31, 2018, previously published as PCT/US2014/433392, filed on Jun. 20, 2014.
Claims priority of provisional application 61/991,866, filed on May 12, 2014.
Claims priority of provisional application 61/943,794, filed on Feb. 24, 2014.
Claims priority of provisional application 61/883,727, filed on Sep. 27, 2013.
Claims priority of provisional application 61/838,650, filed on Jun. 24, 2013.
Prior Publication US 2023/0044835 A1, Feb. 9, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C11B 13/00 (2006.01); B01D 3/00 (2006.01); C11B 3/00 (2006.01); C12N 9/28 (2006.01); C12N 9/34 (2006.01); C12N 9/50 (2006.01); C12N 9/52 (2006.01); C12N 9/58 (2006.01); C12P 7/10 (2006.01); C12P 7/64 (2022.01)
CPC C11B 13/00 (2013.01) [C11B 3/003 (2013.01); C12N 9/2417 (2013.01); C12N 9/2428 (2013.01); C12N 9/50 (2013.01); C12N 9/52 (2013.01); C12N 9/58 (2013.01); C12P 7/10 (2013.01); C12P 7/64 (2013.01); B01D 3/002 (2013.01); C12Y 302/01001 (2013.01); C12Y 302/01003 (2013.01); C12Y 304/00 (2013.01); Y02E 50/10 (2013.01)] 24 Claims
 
1. A process of recovering oil, comprising:
(a) liquefying a starch-containing material into dextrins with a bacterial alpha-amylase at a temperature above the initial gelatinization temperature, wherein the starch-containing material comprises corn;
(b) saccharifying the dextrins using a glucoamylase to form a sugar;
(c) fermenting the sugar in a fermentation medium into ethanol using a yeast fermenting organism;
(d) recovering the fermentation product to form a whole stillage;
(e) separating the whole stillage into thin stillage and wet cake;
(e′) optionally concentrating the thin stillage into syrup; and
(f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease having at least 60% sequence identity to the amino acid sequence of SEQ ID NO: 4 is added during step (a).