US 11,939,363 B2
Translatable molecules and synthesis thereof
Pattraranee Limphong, San Diego, CA (US); Kiyoshi Tachikawa, San Diego, CA (US); Padmanabh Chivukula, San Diego, CA (US); Daiki Matsuda, San Diego, CA (US); and Arisa Cale, San Diego, CA (US)
Assigned to ARCTURUS THERAPEUTICS, INC., San Diego, CA (US)
Filed by ARCTURUS THERAPEUTICS, INC., San Diego, CA (US)
Filed on Jul. 14, 2022, as Appl. No. 17/812,576.
Application 17/812,576 is a continuation of application No. 15/907,123, filed on Feb. 27, 2018, granted, now 11,407,800.
Claims priority of provisional application 62/465,073, filed on Feb. 28, 2017.
Prior Publication US 2023/0059111 A1, Feb. 23, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/505 (2006.01); A61K 31/7115 (2006.01); A61K 38/17 (2006.01); A61K 38/18 (2006.01); A61K 38/48 (2006.01); C07K 14/575 (2006.01); C07K 14/745 (2006.01); C07K 14/81 (2006.01); A61K 38/00 (2006.01); C12Q 1/68 (2018.01)
CPC C07K 14/505 (2013.01) [A61K 31/7115 (2013.01); A61K 38/1709 (2013.01); A61K 38/1816 (2013.01); A61K 38/482 (2013.01); A61K 38/4846 (2013.01); C07K 14/575 (2013.01); C07K 14/745 (2013.01); C07K 14/81 (2013.01); A61K 38/00 (2013.01)] 20 Claims
 
1. A composition comprising a modified messenger RNA (mRNA), wherein
(i) the modified mRNA comprises a coding region of a wild-type mRNA that is expressible, wherein
(a) one or more codons of the coding region have been replaced such that the occurrence of uridine monomers in the coding region is reduced by at least 20% as compared to the coding region of the wild type mRNA; and
(b) the modified mRNA encodes a polypeptide or protein; and
(ii) modified mRNA contains one or more 5-methoxyuridines,
wherein the composition comprises an at least three-fold lower level of double stranded RNA as compared to a composition comprising an mRNA having the same coding region sequence with a similarly reduced occurrence of uridine monomers in the coding region as the modified mRNA and lacking 5-methoxyuridine, and
wherein the composition is a synthesis mixture.