US 11,939,356 B2
Self-assembling insect ferritin nanoparticles
Peter Kwong, Washington, DC (US); Ivelin Georgiev, Nashville, TN (US); Michael Gordon Joyce, Washington, DC (US); Masaru Kanekiyo, Chevy Chase, MD (US); Aliaksandr Druz, Germantown, MD (US); Ulrich Baxa, Frederick, MD (US); Joseph Van Galen, North Wales, PA (US); Cheng Cheng, Rockville, MD (US); John Mascola, Rockville, MD (US); Yaroslav Tsybovsky, Brunswick, MD (US); Yongping Yang, Potomac, MD (US); Barney Graham, Rockville, MD (US); Syed Mohammad Moin, Bethesda, MD (US); and Jeffrey Boyington, Clarksburg, MD (US)
Assigned to The United States of America, as represented by the Secretary, Department of Health and Human Services, Bethesda, MD (US)
Filed by The United States of America, as represented by the Secretary, Department of Health and Human Services, Bethesda, MD (US)
Filed on Mar. 15, 2021, as Appl. No. 17/202,231.
Application 17/202,231 is a division of application No. 16/312,166, granted, now 10,961,283, previously published as PCT/US2017/039595, filed on Jun. 27, 2017.
Claims priority of provisional application 62/355,212, filed on Jun. 27, 2016.
Prior Publication US 2021/0198324 A1, Jul. 1, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 14/11 (2006.01); A61K 39/21 (2006.01); C07K 14/08 (2006.01); C07K 14/16 (2006.01); C07K 14/165 (2006.01); C07K 14/435 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)
CPC C07K 14/11 (2013.01) [A61K 39/21 (2013.01); C07K 14/082 (2013.01); C07K 14/162 (2013.01); C07K 14/165 (2013.01); C07K 14/43563 (2013.01); C12N 7/00 (2013.01); A61K 2039/55555 (2013.01); C07K 2319/735 (2013.01); C12N 2740/16134 (2013.01); C12N 2760/16134 (2013.01); C12N 2770/20034 (2013.01)] 19 Claims
 
1. A recombinant insect ferritin nanoparticle, comprising:
twelve recombinant ferritin heavy chains and twelve recombinant ferritin light chains self-assembled into a globular ferritin nanoparticle; wherein
(A)
the recombinant insect ferritin heavy chains consist of 172 to 174 amino acids from the C-terminus of an insect ferritin heavy chain;
the recombinant insect ferritin light chains consist of 182 to 184 amino acids or 177 amino acids from the C-terminus of an insect ferritin light chain; and
each of the ferritin heavy and light chains is fused N terminally to a viral protein; and
(B)
the recombinant ferritin heavy chains consist of an amino acid sequence at least 90% identical to SEQ ID NO: 2; and the recombinant ferritin light chains consist of an amino acid sequence at least 90% identical to SEQ ID NO: 6; or
the recombinant ferritin heavy chains each consist of an amino acid sequence at least 90% identical to SEQ ID NO: 4; and the recombinant ferritin light chains each consist of an amino acid sequence at least 90% identical to SEQ ID NO: 8.