	US 11,938,475 B2
	Flow cells with hydrogel coating
Hongji Ren, San Diego, CA (US); Jonathan Mark Boutell, Bishops Stortford (GB); John A. Moon, San Diego, CA (US); M. Shane Bowen, Encinitas, CA (US); Alex Nemiroski, San Diego, CA (US); Gary Mark Skinner, Kedington (GB); and Kenny Chen, San Diego, CA (US)
Assigned to Ilumina, Inc., San Diego, CA (US); and Illumina Cambridge Limited, Cambridge (GB)
Filed by ILLUMINA, INC., San Diego, CA (US); and ILLUMINA CAMBRIDGE LIMITED, Cambridge (GB)
Filed on Dec. 7, 2020, as Appl. No. 17/114,134.
Application 17/114,134 is a division of application No. 16/626,393, granted, now 10,919,033, previously published as PCT/US2018/066011, filed on Dec. 17, 2018.
Claims priority of provisional application 62/609,105, filed on Dec. 21, 2017.
Prior Publication US 2021/0086174 A1, Mar. 25, 2021
Int. Cl. B01L 3/00 (2006.01); B01J 19/00 (2006.01); C08F 220/56 (2006.01); C08F 220/60 (2006.01); C09D 133/26 (2006.01); C12Q 1/6876 (2018.01)

CPC B01L 3/502 (2013.01) [B01J 19/0046 (2013.01); C08F 220/56 (2013.01); C08F 220/603 (2020.02); C09D 133/26 (2013.01); C12Q 1/6876 (2013.01); B01J 2219/00475 (2013.01); B01J 2219/00729 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0893 (2013.01); B01L 2300/16 (2013.01)]

7 Claims

1. A method, comprising:

applying a functionalized coating layer in depressions of a patterned flow cell substrate, wherein the depressions are separated by interstitial regions and wherein the patterned flow cell substrate includes a bonding region;

grafting a primer to the functionalized coating layer to form a grafted functionalized coating layer in the depressions;

applying a non-grafted hydrogel on at least the grafted functionalized coating layer, the non-grafted hydrogel being selected from the group consisting of crosslinked polyacrylamide, an agarose gel, and crosslinked polyethylene glycol; and

bonding a lid to the patterned flow cell substrate at the bonding region, thereby defining a flow channel between the patterned flow cell substrate and the lid.