	US 11,938,247 B2
	Human nasal turbinate-derived mesenchymal stem cell-based, 3D bioprinted construct, and use thereof
Sung Won Kim, Seoul (KR); Jung Yeon Lim, Seoul (KR); Sun Hwa Park, Seoul (KR); Byeong Gon Yoon, Suwon-si (KR); Dong-Woo Cho, Seoul (KR); Jinah Jang, Pohang-si (KR); and Seok Won Kim, Seoul (KR)
Assigned to CATHOLIC UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION, Seoul (KR)
Appl. No. 16/609,887
Filed by CATHOLIC UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION, Seoul (KR)
PCT Filed May 2, 2018, PCT No. PCT/KR2018/005068 § 371(c)(1), (2) Date Oct. 31, 2019, PCT Pub. No. WO2018/203651, PCT Pub. Date Nov. 8, 2018.
Claims priority of application No. 10-2017-0056451 (KR), filed on May 2, 2017; and application No. 10-2018-0050195 (KR), filed on Apr. 30, 2018.
Prior Publication US 2020/0197570 A1, Jun. 25, 2020
Int. Cl. A61L 27/38 (2006.01); A61L 27/20 (2006.01); A61L 27/24 (2006.01); A61L 27/52 (2006.01); A61L 27/54 (2006.01)

CPC A61L 27/3834 (2013.01) [A61L 27/20 (2013.01); A61L 27/24 (2013.01); A61L 27/52 (2013.01); A61L 27/54 (2013.01); A61L 2400/06 (2013.01)]

3 Claims

1. A method for fabricating a human nasal turbinate-derived mesenchymal stem cell-based 3D bioprinted stem cell construct, the method comprising the steps of:

(a) isolating mesenchymal stem cells from a nasal turbinate tissue collected during the process of surgically removing the human nasal turbinate;

(b) fabricating a bio-ink by encapsulating the isolated mesenchymal stem cells in a hydrogel; and

(c) fabricating a 3D bioprinted stem cell construct by micro-extruding the bio-ink together with polycaprolactone (PCL) using a multihead dispenser,

wherein the human nasal turbinate-derived mesenchymal stem cell-based 3D bioprinted stem cell construct obtained has bone defect regeneration ability, and

wherein the human nasal turbinate-derived mesenchymal stem cell is differentiated into osteocytes.