US 11,938,221 B2
Multivalent nanoparticle-based vaccines
Barney S. Graham, Rockville, MD (US); Masaru Kanekiyo, Chevy Chase, MD (US); and Hadi M. Yassine, Boyds, MD (US)
Assigned to The United States of America, as represented by the Secretary, Department of Health and Human Services, Bethesda, MD (US)
Filed by The United States of America, as represented by the Secretary, Department of Health and Human Services, Bethesda, MD (US)
Filed on Nov. 4, 2021, as Appl. No. 17/519,142.
Application 17/519,142 is a division of application No. 15/540,898, granted, now 11,191,727, previously published as PCT/US2015/068272, filed on Dec. 31, 2015.
Claims priority of provisional application 62/098,755, filed on Dec. 31, 2014.
Prior Publication US 2022/0054419 A1, Feb. 24, 2022
Int. Cl. A61K 39/12 (2006.01); A61K 9/16 (2006.01); A61K 39/145 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)
CPC A61K 9/167 (2013.01) [A61K 39/12 (2013.01); A61K 39/145 (2013.01); C12N 7/00 (2013.01); A61K 2039/575 (2013.01); A61K 2039/6031 (2013.01); A61K 2039/6068 (2013.01); A61K 2039/6075 (2013.01); A61K 2039/70 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16171 (2013.01); Y02A 50/30 (2018.01)] 8 Claims
 
1. A method of eliciting in an individual, a broadly neutralizing immune response against influenza virus, comprising administering to the individual a nanoparticle comprising:
at least two species of self-assembling fusion proteins, each fusion protein comprising a self-assembling, monomeric subunit protein joined to an immunogenic portion of an influenza virus hemagglutinin (HA) protein, wherein the immunogenic portion of the influenza virus HA protein is a receptor binding domain (RBD);
wherein the immunogenic portion in each species of self-assembling fusion protein differs from the immunogenic portion in the other species of self-assembling fusion proteins by at least one amino acid;
wherein the nanoparticle displays on its surface, the immunogenic portions of the at least two species of self-assembling fusion proteins; and
wherein at least one of the species of self-assembling fusion proteins comprises an amino acid sequence at least 80% identical to SEQ ID NO: 97.