CPC A61K 9/167 (2013.01) [A61K 39/12 (2013.01); A61K 39/145 (2013.01); C12N 7/00 (2013.01); A61K 2039/575 (2013.01); A61K 2039/6031 (2013.01); A61K 2039/6068 (2013.01); A61K 2039/6075 (2013.01); A61K 2039/70 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16171 (2013.01); Y02A 50/30 (2018.01)] | 8 Claims |
1. A method of eliciting in an individual, a broadly neutralizing immune response against influenza virus, comprising administering to the individual a nanoparticle comprising:
at least two species of self-assembling fusion proteins, each fusion protein comprising a self-assembling, monomeric subunit protein joined to an immunogenic portion of an influenza virus hemagglutinin (HA) protein, wherein the immunogenic portion of the influenza virus HA protein is a receptor binding domain (RBD);
wherein the immunogenic portion in each species of self-assembling fusion protein differs from the immunogenic portion in the other species of self-assembling fusion proteins by at least one amino acid;
wherein the nanoparticle displays on its surface, the immunogenic portions of the at least two species of self-assembling fusion proteins; and
wherein at least one of the species of self-assembling fusion proteins comprises an amino acid sequence at least 80% identical to SEQ ID NO: 97.
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