	US 11,938,187 B2
	Methods and compositions for synthesis of two-photon cleavable phosphoramidite molecules for oligonucleotide conjugation
Peggy Pik Kwan Lo, Kowloon (HK); Ling Sum Liu, Kowloon (HK); and Dick Yan Tam, Kowloon (HK)
Assigned to CITY UNIVERSITY OF HONG KONG, Kowloon (HK)
Filed by City University of Hong Kong, Kowloon (HK)
Filed on Jun. 10, 2021, as Appl. No. 17/344,920.
Prior Publication US 2023/0000983 A1, Jan. 5, 2023
Int. Cl. A61K 41/00 (2020.01); A61K 31/713 (2006.01); A61K 47/54 (2017.01); C07F 9/24 (2006.01)

CPC A61K 41/0042 (2013.01) [A61K 31/713 (2013.01); A61K 47/54 (2017.08); C07F 9/242 (2013.01)]

3 Claims

1. A conjugated molecule comprising a photocleavable group linked to a nucleic acid, wherein

the photocleavable group is a 4,4′-bis-(8-(4-(nitro-3-(2-propyl)-styryl))-3,3′-dimethoxybiphenyl (BNSMB) group conferred by 2-(5-((E)-2-(4′-((E)-3-(1-bis(4-methoxyphenyl)(phenyl)methoxy)propan-2-yl)-4-nitrostyryl)-3.3′-dimethoxy-[1,1′-biphenyl]-4-yl)vinyl)-2-nitrophenyl)propyl(2-cyanoethyl) diisopropylphosphoramidite coupled to the nucleic acid during synthesis of the conjugated molecule; and

the conjugated molecule is capable of releasing the nucleic acid therefrom upon being exposed to near infrared (NIR) light to cleave away the photocleavable group.