CPC A61K 39/13 (2013.01) [C12N 7/00 (2013.01); A61K 2039/5258 (2013.01); A61K 2039/53 (2013.01); C12N 2770/32623 (2013.01); C12N 2770/32634 (2013.01)] | 11 Claims |
1. A poliovirus-like particle (VLP) which comprises modifications relative to the poliovirus particle from which the VLP is derived which stabilise the VLP, wherein:
(a) the VLP is derived from a type 1 poliovirus and comprises the following mutations: R4018G, T2025A, D2057E, L3119M, Q3178L and H1248P;
(b) the VLP is derived from a type 2 poliovirus and comprises the following mutations: L3085F, Q3178L, T1041I, F1134L and Y1159F;
(c) the VLP is derived from a type 2 poliovirus and comprises the following mutations: I4057V, D2057A, Q3178L, V1107I, F1134L and V1183L; or
(d) the VLP is derived from a type 3 poliovirus and comprises the following mutations: T4067A, L20181, L2215M, D2241E, H3019Y, L3085F, T1105M and F1132L;
wherein the modifications relative to the poliovirus particle from which the VLP is derived stabilises the native-antigenic conformation of the poliovirus capsid.
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