US 11,937,951 B2
Method and apparatus for medical imaging using differencing of multiple fluorophores
Kenneth Tichauer, Chicago, IL (US); Robert W. Holt, Lebanon, NH (US); Frederic Leblond, Montreal (CA); Pablo Valdes, Hanover, NH (US); Brian W. Pogue, Hanover, NH (US); Keith D. Paulsen, Hanover, NH (US); and David W. Roberts, Lyme, NH (US)
Assigned to The Trustees of Dartmouth College, Hanover, NH (US)
Filed by The Trustees of Dartmouth College, Hanover, NH (US)
Filed on Jan. 25, 2023, as Appl. No. 18/159,495.
Application 18/159,495 is a continuation of application No. 14/767,836, granted, now 11,564,639, previously published as PCT/US2014/016291, filed on Feb. 13, 2014.
Claims priority of provisional application 61/764,506, filed on Feb. 13, 2013.
Prior Publication US 2023/0233157 A1, Jul. 27, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. A61B 5/00 (2006.01); A61K 49/00 (2006.01)
CPC A61B 5/7278 (2013.01) [A61B 5/0071 (2013.01); A61K 49/0058 (2013.01); A61B 5/0035 (2013.01); A61B 5/0062 (2013.01); A61B 2576/00 (2013.01)] 15 Claims
OG exemplary drawing
 
1. A method of generating corrected fluorescence data of concentrations of a targeted fluorophore in tissue of a subject, comprising:
administering a first fluorescent contrast agent to the subject, the first fluorescent contrast agent selected from the group consisting of tissue-targeted agents containing the targeted fluorophore and agents containing a tissue-targeted prodrug metabolizable into the targeted fluorophore;
administering a second fluorescent contrast agent to the subject, the second fluorescent contrast agent having tissue affinity differing from a tissue affinity of the first fluorescent contrast agent and containing a second fluorophore;
illuminating tissue of the subject with light of a first stimulus wavelength;
acquiring first data at a first emissions wavelength associated with the targeted fluorophore;
illuminating tissue of the subject with light of a second stimulus wavelength;
acquiring second data at a second emissions wavelength associated with the second fluorophore, the second emissions wavelength different from the first emissions wavelength; and
subtracting the second data from the first data to provide the corrected fluorescence data;
wherein the targeted fluorophore emits light of the first emissions wavelength when stimulated by light of the first stimulus wavelength, and the second fluorophore emits light of the second emissions wavelength when stimulated by light of the second stimulus wavelength; and
reconstructing bound fluorophore concentrations of a voxel-based three dimensional model of distribution of bound targeted fluorophore concentration in tissue from the corrected fluorescence data.