US 11,937,586 B2
Compositions and methods for producing genetically modified animals
Frank Koentgen, North Beach (AU)
Assigned to OZGENE HOLDINGS PTY LTD, Bentley (AU)
Filed by OZGENE HOLDINGS PTY LTD., Bentley (AU)
Filed on Nov. 30, 2021, as Appl. No. 17/538,206.
Application 17/538,206 is a division of application No. 15/034,970, previously published as PCT/AU2014/050339, filed on Nov. 7, 2014.
Claims priority of application No. 2013904307 (AU), filed on Nov. 7, 2013; and application No. 2014902162 (AU), filed on Jun. 6, 2014.
Prior Publication US 2022/0201992 A1, Jun. 30, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01K 67/027 (2006.01); A01K 67/0271 (2024.01); A01K 67/0276 (2024.01); C12N 5/0735 (2010.01); C12N 15/85 (2006.01)
CPC A01K 67/0276 (2013.01) [A01K 67/0271 (2013.01); C12N 5/0606 (2013.01); C12N 15/8509 (2013.01); A01K 2207/12 (2013.01); A01K 2217/075 (2013.01); A01K 2227/105 (2013.01); A01K 2267/025 (2013.01); C12N 2510/00 (2013.01); C12N 2800/30 (2013.01)] 12 Claims
 
1. A method of producing male mouse embryos, wherein each and every male mouse embryo produced is infertile, the method comprising:
crossing: 1) a first mouse breeding partner carrying a disruptable transgene that comprises a GILZ gene that is operably connected to a promoter and to recombinase recognition sites that mediate disruption of the GILZ gene in the presence of a recombinase, wherein disruption of the GILZ gene has been predetermined to lead to male infertility in a mouse of a genetic background, and wherein the first mouse breeding partner is female and is homozygous for the disruptable transgene;
with 2) a second mouse breeding partner carrying a disruptor transgene that comprises a disruptor nucleotide sequence that is operably connected to a promoter and that encodes the recombinase that mediates disruption of the GILZ gene of the disruptable transgene, wherein the second mouse breeding partner is male and is homozygous for the disruptor transgene,
to thereby generate a male mouse embryo that comprises germ cells having the GILZ gene disrupted therein, wherein mouse embryos produced from the crossing are of the genetic background in which disruption of the GILZ gene has been predetermined to lead to male infertility, and wherein each and every male mouse embryo produced from the crossing is infertile; and
isolating the male mouse embryo.